Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan, 410082, China.
State Key Laboratory Base of Novel Functional Materials and Preparation Science, Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, School of Materials Science and Chemical Engineering, Institute of Mass Spectrometry, Ningbo University, Ningbo, Zhejiang, 315211, China.
Angew Chem Int Ed Engl. 2021 May 25;60(22):12569-12576. doi: 10.1002/anie.202103027.
The novel theranostic nanosystems based on two-photon fluorescence can achieve higher spatial resolution of deep tissue imaging for simultaneous diagnosis and therapy of a variety of cancers. Herein, we have designed and prepared FRET-based two-photon mesoporous silica nanoparticles (MTP-MSNs) for single-excitation multiplexed intracellular imaging and targeted cancer therapy for the first time. This nanosystem includes two constituents, containing (1) multicolor two-photon mesoporous silica nanoparticles and (2) cancer cell-targeting aptamers that act as gatekeepers for MTP-MSNs. After incubation with cancer cells, the Dox-loaded and aptamer-capped MTP-MSNs could be internalized into the cells, opening the pores and releasing the drug. Furthermore, using two-photon multicolor fluorescence, MTP-MSNs could serve as good contrast agents for multicolor two-photon intracellular imaging with increased imaging depth and improved spatial localization of tissue. In sum, these multicolor MTP-MSNs provide a promising system for traceable targeted cancer therapy with further applications in multiplex intracellular imaging and the screening of drug.
基于双光子荧光的新型治疗性纳米系统可实现更深组织成像的更高空间分辨率，从而对各种癌症进行同时诊断和治疗。在此，我们首次设计并制备了基于 FRET 的双光子介孔硅纳米颗粒（MTP-MSNs），用于单激发的多重细胞内成像和靶向癌症治疗。该纳米系统包含两个组成部分，包括（1）多色双光子介孔硅纳米颗粒和（2）作为 MTP-MSN 门控的癌细胞靶向适体。与癌细胞孵育后，负载 Dox 的和适体封闭的 MTP-MSNs 可被内化进入细胞，打开孔并释放药物。此外，使用双光子多色荧光，MTP-MSNs 可用作多色双光子细胞内成像的良好造影剂，增加了成像深度并改善了组织的空间定位。总之，这些多色 MTP-MSNs 为可追踪的靶向癌症治疗提供了有前景的系统，可进一步应用于多重细胞内成像和药物筛选。
