Aguila H N, Pakes S P, Lai W C, Lu Y S
Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235.
Lab Anim Sci. 1988 Apr;38(2):148-51.
Splenic natural killer cell activity and plasma corticosterone levels were measured in air- and truck-transported C57BL/6J mice (Mus musculus) on days 0, 1, 3 and 5 post-arrival. These data are important in determining adequate stabilization periods for transported animals before studies involving natural killer cells are begun. Three control groups (phosphate buffered saline, polyinosinic-polycytidylic acid, and hydrocortisone injected mice) were stabilized in the animal facilities 3 weeks before the start of experiments. Natural killer activity in transported mice was reduced significantly (p less than 0.05) on day 0 and returned to normal levels by 24 hours. Plasma corticosterone levels were increased significantly (p less than 0.005) on day 0 and returned to control levels by day 1, correlating inversely with splenic natural killer activity. This study indicates that stress resulting from transportation causes a short-term decrease in the splenic natural killer cell activity of mice, and this decrease may be related to the increased plasma corticosterone levels induced by the stressful event. We conclude that mice should be stabilized at least 24 hours before experiments involving the natural killer cell system are begun.
在到达后的第0、1、3和5天，对通过空运和卡车运输的C57BL/6J小鼠（小家鼠）的脾脏自然杀伤细胞活性和血浆皮质酮水平进行了测量。这些数据对于在开始涉及自然杀伤细胞的研究之前确定运输动物的适当稳定期很重要。三个对照组（注射磷酸盐缓冲盐水、聚肌苷酸-聚胞苷酸和氢化可的松的小鼠）在实验开始前3周在动物设施中进行稳定处理。运输小鼠的自然杀伤活性在第0天显著降低（p<0.05），并在24小时内恢复到正常水平。血浆皮质酮水平在第0天显著升高（p<0.005），并在第1天恢复到对照水平，与脾脏自然杀伤活性呈负相关。本研究表明，运输引起的应激导致小鼠脾脏自然杀伤细胞活性短期下降，这种下降可能与应激事件诱导的血浆皮质酮水平升高有关。我们得出结论，在开始涉及自然杀伤细胞系统的实验之前，小鼠应至少稳定24小时。
