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Noninvasive preimplantation genetic testing for aneuploidy in spent culture medium as a substitute for trophectoderm biopsy.

作者信息

Rubio Carmen, Racowsky Catherine, Barad David H, Scott Richard T, Simon Carlos

机构信息

Igenomix, Research and Development, Valencia, Spain; Igenomix Foundation/INCLIVA, Reproductive Genetics, Valencia, Spain.

Department of Obstetrics, Gynecology and Reproductive Medicine, Hôpital Foch, Suresnes, France.

出版信息

Fertil Steril. 2021 Apr;115(4):841-849. doi: 10.1016/j.fertnstert.2021.02.045. Epub 2021 Mar 17.

DOI:10.1016/j.fertnstert.2021.02.045
PMID:33741125
Abstract
摘要

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Noninvasive preimplantation genetic testing for aneuploidy in spent culture medium as a substitute for trophectoderm biopsy.利用废弃培养基进行非侵入性胚胎植入前非整倍体基因检测以替代滋养外胚层活检
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Noninvasive preimplantation genetic testing for aneuploidy in spent blastocyst media will substitute for trophectoderm biopsy.对废弃囊胚培养液进行非侵入性植入前非整倍体基因检测将替代滋养外胚层活检。
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Multicenter prospective study of concordance between embryonic cell-free DNA and trophectoderm biopsies from 1301 human blastocysts.多中心前瞻性研究 1301 个人类囊胚的胚胎游离 DNA 与滋养层活检的一致性。
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Noninvasive preimplantation genetic testing for aneuploidy in spent medium may be more reliable than trophectoderm biopsy.在已使用的培养液中进行非侵入性胚胎植入前遗传学检测（PGT-A）来筛查非整倍体可能比滋养外胚层活检更可靠。
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Clinical application of embryo aneuploidy testing by next-generation sequencing.基于新一代测序的胚胎非整倍体检测的临床应用。
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Culture time to optimize embryo cell-free DNA analysis for frozen-thawed blastocysts undergoing noninvasive preimplantation genetic testing for aneuploidy.优化胚胎无细胞 DNA 分析的培养时间，用于对进行非整倍体无创性植入前遗传学检测的冷冻解冻囊胚。
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Non-invasive preimplantation genetic testing (niPGT): the next revolution in reproductive genetics?非侵入性胚胎植入前遗传学检测（niPGT）：生殖遗传学的下一次革命？
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Cell-free DNA in spent culture medium effectively reflects the chromosomal status of embryos following culturing beyond implantation compared to trophectoderm biopsy.与滋养层活检相比，培养超过着床期后，废弃培养液中的游离 DNA 能更有效地反映胚胎的染色体状态。
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Re-denudation of residual cumulus cells on day 3 increases the accuracy of cell-free DNA detection in spent embryo culture medium.第三天重新剥除剩余卵丘细胞可提高胚胎培养液中游离 DNA 检测的准确性。
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Concordance of PGT for aneuploidies between blastocyst biopsies and spent blastocyst culture medium.囊胚活检与废弃囊胚培养基之间非整倍体植入前基因检测的一致性
Reprod Biomed Online. 2023 Mar;46(3):483-490. doi: 10.1016/j.rbmo.2022.10.001. Epub 2022 Oct 12.

引用本文的文献

1
Current research status and clinical applications of noninvasive preimplantation genetic testing: A review.当前无创性胚胎植入前遗传学检测的研究现状与临床应用：综述
Medicine (Baltimore). 2024 Oct 4;103(40):e39964. doi: 10.1097/MD.0000000000039964.
2
Association of early cleavage, morula compaction and blastocysts ploidy of IVF embryos cultured in a time-lapse system and biopsied for genetic test for aneuploidy.体外受精胚胎在时间延迟系统中培养并进行用于非整倍体遗传检测的活检后，其早期卵裂、桑椹胚致密和囊胚倍性的相关性。
Sci Rep. 2024 Jan 7;14(1):739. doi: 10.1038/s41598-023-51087-z.
3
Effectiveness of non-invasive chromosomal screening for normal karyotype and chromosomal rearrangements.
无创染色体筛查对正常核型和染色体重排的有效性。
Front Genet. 2023 Mar 13;14:1036467. doi: 10.3389/fgene.2023.1036467. eCollection 2023.
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Preimplantation Genetic Testing for Monogenic Conditions: Is Cell-Free DNA Testing the Next Step?胚胎植入前遗传学检测：单基因疾病的检测——游离 DNA 检测是否是下一个步骤？
Mol Diagn Ther. 2021 Nov;25(6):683-690. doi: 10.1007/s40291-021-00556-0. Epub 2021 Sep 8.