Kong Lingbo, Ma Rui, Cao Yang, Smith Wanli, Liu Yuan, Yang Xiaobin, Yan Liang
Department of Spine Surgery, School of Medicine, Honghui Hospital, Xi'an Jiaotong University, Xi'an, China.
Department of Anesthesiology, Xi'an Children Hospital, Xi'an, China.
J Cell Mol Med. 2021 May;25(10):4649-4657. doi: 10.1111/jcmm.16390. Epub 2021 Mar 19.
Although document studies (including ours) have been reported the achieved in vitro osteoclastic cellular model establishment from the RAW264.7 cell lineage, there was no study directly reported that American Type Culture Collection (ATCC) cell bank has various RAW264.7 cell lineages. Besides that, for our knowledge there was only one study compared the two different RAW264.7 and RAW264.7 cell lineages for their osteoclastic differentiation, and they concluded that the RAW264.7 demonstrated to generate much osteoclast than RAW264.7 . However, on the contrary to their results we noticed the fusion of RAW264.7 in our previous studies was much compromising. Therefore, we try to explore the two cell lineages for their properties in osteoclastic differentiation with an in-depth cellular cytoskeletal study. Our current study has showed that comparing to the RAW264.7 , RAW264.7 demonstrated a much higher efficacies for RANKL-stimulated osteoclastic differentiation. Besides that, in our depth cytoskeletal studies, we found that the RANKL-induced RAW264.7 cells could finally differentiate into mature osteoclasts. However, regardless the various pre-treatment conditions, there was no mature osteoclast formed in RANKL-induced RAW264.7 cell lineage.
尽管已有文献研究（包括我们的研究）报道了从RAW264.7细胞系成功建立体外破骨细胞模型，但尚无研究直接报道美国典型培养物保藏中心（ATCC）细胞库有多种RAW264.7细胞系。除此之外，据我们所知，仅有一项研究比较了两种不同的RAW264.7细胞系在破骨细胞分化方面的差异，他们得出结论，RAW264.7比RAW264.7产生的破骨细胞更多。然而，与他们的结果相反，我们在之前的研究中注意到RAW264.7的融合情况较差。因此，我们试图通过深入的细胞骨架研究来探索这两种细胞系在破骨细胞分化方面的特性。我们目前的研究表明，与RAW264.7相比，RAW264.7在RANKL刺激下的破骨细胞分化效率更高。除此之外，在我们深入的细胞骨架研究中，我们发现RANKL诱导的RAW264.7细胞最终可分化为成熟破骨细胞。然而，无论预处理条件如何，RANKL诱导的RAW264.7细胞系中均未形成成熟破骨细胞。
