Ge Yi, Xu Min-Min, Xu Shao-Hui, Xu Yan
School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China.
Shandong Academy of Environmental Sciences Company Limited, Ji'nan 250100, China.
Huan Jing Ke Xue. 2021 Feb 8;42(2):996-1003. doi: 10.13227/j.hjkx.202005252.
Wheat is the main food crop in China while at the same time, heavy metals pose a significant threat to crop growth and food security. Many studies indicate that rhizospheric microorganism play an important role in regulating crop development and stress resistance. In this study, the variation in wheat root-associated microbial communities under copper pollution was studied using high-throughput sequencing. The microbial community structure and diversity among different wheat rhizocompartments were compared after sequencing of microbial communities in the bulk soil, rhizosphere, and endosphere of wheat under copper pollution in combination with pot-based experiments. The results showed that the microbial diversity of the endosphere was significantly lower than in the rhizosphere and bulk soil(<0.001), indicating that root surfaces serve as a gateway for microorganisms to enter into the interior root environment, and play a role in filtering root colonization. Copper pollution significantly reduced the microbial diversity of the rhizosphere (<0.05). In the bulk soil and endosphere environments, although copper pollution reduced microbial diversity in the corresponding rhizocompartment, the difference was not significant (>0.05). Proteobacteria and Actinobacteria were the dominant bacteria groups in the rhizosphere and the bulk soil under copper pollution. In addition, microbes such as , and show strong stress resistance and can provide nutrients for plants.
小麦是中国的主要粮食作物，与此同时，重金属对作物生长和粮食安全构成重大威胁。许多研究表明，根际微生物在调节作物发育和抗逆性方面发挥着重要作用。在本研究中，利用高通量测序技术研究了铜污染下小麦根际微生物群落的变化。结合盆栽试验，对铜污染下小麦的根际土壤、根际和内生菌中的微生物群落进行测序后，比较了不同小麦根际隔室间的微生物群落结构和多样性。结果表明，内生菌的微生物多样性显著低于根际和根际土壤（<0.001），这表明根表面是微生物进入根内部环境的通道，并在筛选根定殖方面发挥作用。铜污染显著降低了根际的微生物多样性（<0.05）。在根际土壤和内生菌环境中，虽然铜污染降低了相应根际隔室的微生物多样性，但差异不显著（>0.05）。变形菌门和放线菌门是铜污染下根际和根际土壤中的优势细菌类群。此外，诸如 、 和 等微生物表现出较强的抗逆性，可为植物提供养分。
