State Key Laboratory of Medicinal Chemical Biology, The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, 300071, China.
The Institute of Robotics and Automatic Information System, Tianjin Key Laboratory of Intelligent Robotics, Nankai University, Tianjin, 300350, China.
Environ Pollut. 2021 Jun 1;278:116887. doi: 10.1016/j.envpol.2021.116887. Epub 2021 Mar 9.
6-benzylaminopurine (6-BA) is one of the first synthetic hormones and has been widely used in fruit cultivation, gardening and agriculture. However, excessive use of 6-BA will cause potential harm to the environment and humans. Therefore, our research focused on assessing the impact of 6-BA on the development and neurobehavior of zebrafish. The results showed that 6-BA had little effect on the embryos from 2 hpf to 10 hpf. However, delayed development, decreased survival and hatchability were observed under 30 and 40 mg/L 6-BA from 24 hpf. 6-BA also reduced surface tension of embryonic chorions at 24 hpf. In addition, 6-BA caused abnormal morphology and promoted the accumulation of oxidative stress. Transcription of genes in connection with development and oxidative stress was also strikingly altered. Results of movement assay showed that zebrafish were less active and their behavior was significantly inhibited under the 20 and 30 mg/L 6-BA treatments. Locomotion-related genes th and mao were down-regulated by gradient, while the transcription of dbh was upregulated at a low concentration (2 mg/L) but decreased as the concentration increased. Moreover, 6-BA exposure caused increased arousal and decreased sleep. Sleep/wake related genes hcrt and hcrtr2 were upregulated, but decreased at 30 mg/L, while the mRNA level of aanat2 was reduced in a concentration-dependent manner. To sum up, our results showed that 6-BA induced developmental toxicity, promoted the accumulation of oxidative stress, and damaged locomotion and sleep/wake behavior.
6-苄基氨基嘌呤（6-BA）是最早的合成激素之一，已广泛应用于水果栽培、园艺和农业。然而，6-BA 的过度使用会对环境和人类造成潜在危害。因此，我们的研究集中在评估 6-BA 对斑马鱼发育和神经行为的影响。结果表明，6-BA 对 2 hpf 至 10 hpf 的胚胎影响不大。然而，在 24 hpf 时，30 和 40 mg/L 的 6-BA 会导致胚胎发育延迟、存活率和孵化率降低。6-BA 还降低了 24 hpf 时胚胎绒毛膜的表面张力。此外，6-BA 导致形态异常，并促进氧化应激的积累。与发育和氧化应激相关的基因的转录也明显改变。运动试验结果表明，在 20 和 30 mg/L 6-BA 处理下，斑马鱼的活动减少，行为明显受到抑制。th 和 mao 等与运动相关的基因呈梯度下调，而 dbh 的转录在低浓度（2 mg/L）时上调，但随着浓度的增加而降低。此外，6-BA 暴露会导致觉醒增加和睡眠减少。睡眠/觉醒相关基因 hcrt 和 hcrtr2 上调，但在 30 mg/L 时下调，而 aanat2 的 mRNA 水平则呈浓度依赖性降低。总之，我们的研究结果表明，6-BA 诱导发育毒性，促进氧化应激的积累，并损害运动和睡眠/觉醒行为。
