College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, Gansu, China; Key Laboratory of Grassland Ecosystem, Gansu Agricultural University, Lanzhou 730070, Gansu, China.
College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, Gansu, China; Key Laboratory of Grassland Ecosystem, Gansu Agricultural University, Lanzhou 730070, Gansu, China; Engineering Research Center for the Resource Utilization of Livestock and Poultry Wastes in Gansu Province, Lanzhou 730070, Gansu, China.
J Hazard Mater. 2021 Aug 5;415:125559. doi: 10.1016/j.jhazmat.2021.125559. Epub 2021 Mar 3.
This study aimed to investigate the effect of swine manure composting with microbial inoculation (MI) and without MI (CK) on heavy metal (Cr, Cd, and Pb) fractions, humic substance (HS), and metabolism pathway. The results showed that MI could passivate the heavy metal Cr and reduce the proportion of exchangeable (EXC) fraction of Cd, but it does not affect the EXC fraction of Pb. Compared to CK, HS, humic acid (HA), and fulvic acid (FA) were significantly increased with MI at the maturity stage. The propagation of Proteobacteria (day 4) and Firmicutes (days 12 and 24) was strengthened with MI. Canonical correlation analysis found that HA and Firmicutes were positively correlated with heavy metal (Cr, Cd, and Pb) residual (RES) fraction, and FA was positively correlated with Proteobacteria. Moreover, MI can significantly increase amino acid metabolism and carbohydrate metabolism by day 4, enhance the metabolism of enzyme families and glycan biosynthesis by day 12, and improve membrane transport. Overall, MI could facilitates the increase in HA and FA content and transfer of heavy metal (Cr, Cd, and Pb) fractions, it particularly helps increase the RES fraction.
本研究旨在探讨添加和不添加微生物接种剂（MI）的猪粪堆肥对重金属（Cr、Cd 和 Pb）形态、腐殖质（HS）和代谢途径的影响。结果表明，MI 可以钝化重金属 Cr，并降低 Cd 的可交换（EXC）形态比例，但对 Pb 的 EXC 形态没有影响。与 CK 相比，MI 在成熟阶段显著增加了 HS、胡敏酸（HA）和富里酸（FA）。MI 增强了 Proteobacteria（第 4 天）和 Firmicutes（第 12 天和第 24 天）的繁殖。典型相关分析发现，HA 和 Firmicutes 与重金属（Cr、Cd 和 Pb）残留（RES）形态呈正相关，而 FA 与 Proteobacteria 呈正相关。此外，MI 可以在第 4 天显著增加氨基酸代谢和碳水化合物代谢，在第 12 天增强酶家族和聚糖生物合成的代谢，并改善膜转运。总体而言，MI 可以促进 HA 和 FA 含量的增加和重金属（Cr、Cd 和 Pb）形态的转化，特别是有助于增加 RES 形态。
