Department of Anesthesiology, Puren Hospital Affiliated to Wuhan University of Science and Technology, Wuhan, China.
Department of Anesthesiology, the Affiliated Suzhou Science and Technology Town Hospital of Nanjing Medical University, Suzhou, China.
BMC Anesthesiol. 2021 Mar 20;21(1):87. doi: 10.1186/s12871-021-01305-w.
Waste anesthetic gases (WAGs) leaked from new-type halogenated inhalational anesthetics such as sevoflurane have been were reported to pose a risk for the health of operating room personnel. The effects of WAGs on peripheral blood lymphocytes, however, remain yet controversial. The present study was undertaken to examine the effects of occupational sevoflurane exposure on the peripheral blood lymphocytes of medical personnel who work in the operating room.
A cohort of 56 medical residents were divided into exposed group (n = 28) and control group (non-exposed group) (n = 28). Gas chromatography was used to measure the concentration of sevoflurane in the medical resident's breathing zone during surgeries under inhalation anesthesia in the exposure group. The gas collection lasted an hour. Peripheral blood lymphocytes were isolated from venous blood, and then apoptosis and cell cycle were analyzed by flow cytometry. EDTA-anticoagulated whole blood was harvested to analyze the lymphocyte subsets by flow cytometry. Immunoglobulins (IgA, IgM, IgG) were quantified by immunoturbidimetry.
The average concentration of sevoflurane in the exposed group was 1.03 ppm with a range from 0.03 ppm to 2.24 ppm. No significant effects were found on the apoptosis rates or cell cycles of peripheral blood lymphocytes in the exposed group relative to the control group (P > 0.05). Similarly, there were no significant differences in the lymphocyte subsets or the levels of immunoglobulins (IgA, IgM, IgG) between the two groups (P > 0.05).
Occupational exposure to low-level sevoflurane has no significant effect on the peripheral blood lymphocytes of operating room staff, but this conclusion needs to be confirmed by multicenter and long-term follow-up studies with large samples.
ChiCTR2000040772 , December 9, 2020 (Retrospective registration).
新型卤代吸入麻醉剂（如七氟醚）逸出的废气（WAGs）已被报道对手术室人员的健康构成风险。然而，WAGs 对周围血淋巴细胞的影响仍存在争议。本研究旨在探讨职业性七氟醚暴露对手术室医务人员外周血淋巴细胞的影响。
将 56 名住院医师分为暴露组（n=28）和对照组（非暴露组）（n=28）。在暴露组中，使用气相色谱法测量吸入麻醉下手术期间医务人员呼吸区七氟醚的浓度。气体采集持续 1 小时。从静脉血中分离外周血淋巴细胞，然后通过流式细胞术分析细胞凋亡和细胞周期。采集 EDTA 抗凝全血，通过流式细胞术分析淋巴细胞亚群。用免疫比浊法定量免疫球蛋白（IgA、IgM、IgG）。
暴露组七氟醚的平均浓度为 1.03ppm，范围为 0.03ppm 至 2.24ppm。与对照组相比，暴露组外周血淋巴细胞的凋亡率和细胞周期无显著差异（P>0.05）。两组间淋巴细胞亚群或免疫球蛋白（IgA、IgM、IgG）水平也无显著差异（P>0.05）。
职业性低水平七氟醚暴露对手术室工作人员外周血淋巴细胞无明显影响，但这一结论需要通过多中心、长期随访、大样本量的研究来证实。
ChiCTR2000040772，2020 年 12 月 9 日（回顾性注册）。
