无金属色谱介质。

Metal-free chromatographic media.

作者信息

Martin M T

出版信息

Methods Enzymol. 1988;158:15-21. doi: 10.1016/0076-6879(88)58044-8.

Abstract

The three major considerations in reducing metal-gel binding are (1) the type of gel to be used, (2) the ionic strength of the eluent, and (3) alkaline reduction of the gel. Each of these parameters should be considered in advance of an experiment. A method is offered for determining the optimal gel type, gel treatment, and eluent for a critical metal-free chromatography experiment.

摘要

减少金属-凝胶结合的三个主要考虑因素为：（1）所用凝胶的类型；（2）洗脱液的离子强度；（3）凝胶的碱还原。在实验前应预先考虑这些参数中的每一个。本文提供了一种方法，用于确定关键的无金属色谱实验的最佳凝胶类型、凝胶处理方法和洗脱液。