樱桃谷鸭含三联基序蛋白32的克隆、分析及抗鸭坦布苏病毒天然免疫应答
Cloning, analysis, and anti-duck Tembusu virus innate immune response of Cherry Valley duck tripartite motif-containing 32.
作者信息
Li Tianxu, Hu Xiaofang, Zhang Tingting, Song Xingdong, Zhang Huihui, Dai Ning, Sheng Wei, Hou Xiaolan, Wei Liangmeng
机构信息
Sino-German Cooperative Research Centre for Zoonosis of Animal Origin of Shandong Province, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an City 271018, Shandong Province, China.
Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, College of Basic Medical Sciences, Shandong First Medical University, Tai'an City 271000, Shandong Province, China.
出版信息
Poult Sci. 2021 May;100(5):101048. doi: 10.1016/j.psj.2021.101048. Epub 2021 Feb 15.
Tripartite motif-containing 32 (TRIM32) is an E3 ubiquitin ligase with multiple functions. In this study, we amplified TRIM32 gene from the Cherry Valley duck, and its cDNA sequence contained an open reading frame of 1,950 bp that encodes 649 amino acids. Duck TRIM32 (duTRIM32) mRNA was expressed in all tissues tested. A series of immune-related genes that were induced by viral infection, including interferon alfa, IL-1β, retinoic acid-inducible gene-I, Mx, and OAS, were regulated by duTRIM32 expression. DuTRIM32 overexpression inhibits duck Tembusu virus (DTMUV) replication in the early stages of viral infection. Knockdown of duTRIM32 expression by siRNA reduced the ability of duck embryo fibroblast cells to mount a type Ⅰ interferon response to DTMUV. Therefore, our results suggest that the duTRIM32-mediated signal pathway plays an essential role in DTMUV infection-induced innate immune response.
含三联基序蛋白32（TRIM32）是一种具有多种功能的E3泛素连接酶。在本研究中，我们从樱桃谷鸭中扩增出TRIM32基因，其cDNA序列包含一个1950bp的开放阅读框，编码649个氨基酸。鸭TRIM32（duTRIM32）mRNA在所有检测组织中均有表达。一系列由病毒感染诱导的免疫相关基因，包括α干扰素、白细胞介素-1β、视黄酸诱导基因-I、Mx蛋白和2'-5'-寡腺苷酸合成酶，受duTRIM32表达的调控。duTRIM32的过表达在病毒感染早期抑制鸭坦布苏病毒（DTMUV）的复制。通过小干扰RNA敲低duTRIM32的表达降低了鸭胚成纤维细胞对DTMUV产生Ⅰ型干扰素反应的能力。因此，我们的结果表明duTRIM32介导的信号通路在DTMUV感染诱导的先天免疫反应中起重要作用。
Zotero 插件