Laboratory of Adsorption and Emission Phenomenon, Institute of Ion-Plasma and Laser Technologies, Tashkent, Uzbekistan.
Eur J Mass Spectrom (Chichester). 2021 Feb;27(1):29-38. doi: 10.1177/14690667211002777. Epub 2021 Mar 21.
This paper demonstrates direct detection of stimulants such as amphetamine, methamphetamine and cocaine spiked with untreated urine and a real world sample using surface ionization mass spectrometry. Spiked samples were analyzed without preliminary chromatographic separation and extraction procedure using the developed method. Moreover, in order to check the analytical capabilities of the method non-extracted real world sample was analyzed. After liquid-liquid extraction, the same sample was analyzed using the method for comparative study. Limit of detection of spiked samples was in the range of 10 pg (10 ng/ml) to 100 pg (100 ng/ml). Linear ranges of samples were two orders of magnitude or more than two orders of magnitude. It was revealed these spiked samples and real world sample can be analyzed without preliminary chromatographic separation and preliminary extraction procedure due to high selectivity of the method and the presence of the indicator lines of studied analytes in the mass spectra. The surface ionization mass spectrometry data was attested by the GC/MS analysis of these samples.
本文展示了使用表面电离质谱法对未经处理的尿液和实际样本中的安非他命、甲基苯丙胺和可卡因等兴奋剂进行直接检测。使用所开发的方法，无需进行初步的色谱分离和提取程序即可分析加标样品。此外，为了检查该方法的分析能力，还对未经提取的实际样本进行了分析。经过液液萃取后，使用该方法对同一样本进行了分析，以进行比较研究。加标样品的检出限在 10pg（10ng/ml）至 100pg（100ng/ml）范围内。样品的线性范围为两个数量级或两个以上数量级。结果表明，由于该方法具有高选择性并且在质谱中存在研究分析物的指示线，因此无需进行初步的色谱分离和初步的提取程序即可对这些加标样品和实际样本进行分析。这些样品的表面电离质谱数据已通过 GC/MS 分析得到证实。
