Department of Development and Rehabilitation of Motor Function, Human Health Sciences, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Department of Motor Function Analysis, Human Health Sciences, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Ultrasound Med Biol. 2021 Jun;47(6):1586-1595. doi: 10.1016/j.ultrasmedbio.2021.02.009. Epub 2021 Mar 18.
The aim of this study was to determine that low-intensity pulsed ultrasound (LIPUS) at an intensity of 140 mW/cm promotes functional and histologic improvements in sciatic nerve crush injury in a rat model and to investigate changes over time in relevant growth factors and receptors, exploring the mechanism of LIPUS in the recovery process after injury. Toe angle in the toe-off phase, regenerative axonal length, myelinated nerve fiber density, diameter of myelinated nerve fiber, axon diameter and myelin sheath thickness were significantly higher in the LIPUS group than in the sham group. Gene and protein expression of brain-derived neurotrophic factor (BDNF) was upregulated in the LIPUS group. In conclusion, LIPUS contributed to rapid functional and histologic improvement and upregulated BDNF expression after sciatic nerve crush injury in rats.
本研究旨在确定强度为 140mW/cm 的低强度脉冲超声（LIPUS）可促进大鼠坐骨神经挤压伤的功能和组织学改善，并随时间推移研究相关生长因子和受体的变化，探索 LIPUS 在损伤后恢复过程中的作用机制。在离地阶段，LIPUS 组的足趾角度、再生轴突长度、有髓神经纤维密度、有髓神经纤维直径、轴突直径和髓鞘厚度均显著高于假手术组。LIPUS 组脑源性神经营养因子（BDNF）的基因和蛋白表达上调。总之，LIPUS 促进了大鼠坐骨神经挤压伤后的快速功能和组织学改善，并上调了 BDNF 的表达。
