Department of Laboratory Animals, Jilin Provincial Key Laboratory of Animal Model, Jilin University, Changchun 130062, Jilin, P.R. China.
Int J Med Sci. 2021 Mar 3;18(8):1877-1885. doi: 10.7150/ijms.52177. eCollection 2021.
miRNAs are a family of short, noncoding RNAs that are involved in many processes in melanoma cells. MITF acts as a master regulator of melanocyte function, development and survival by modulating various genes. Hydroxyurea (HU) is used to treat melanoma, and miRNA expression is altered after HU treatment in B16 melanoma cells. In this study, we screened for miRNAs that were upregulated after HU treatment and that targeted the MITF gene. We found that miR-7013-3p exhibited increased expression after HU treatment and could bind to MITF. miR-7013-3p inhibited melanin production, proliferation, and migration and promoted apoptosis in B16 melanoma cells. The results may provide more information on the roles of miR-7013-3p in B16 melanoma cells.
miRNAs 是一类短的非编码 RNA，参与黑色素瘤细胞中的许多过程。MITF 通过调节各种基因，作为黑色素细胞功能、发育和存活的主调控因子发挥作用。羟基脲 (HU) 用于治疗黑色素瘤，HU 处理后 B16 黑色素瘤细胞中的 miRNA 表达发生改变。在本研究中，我们筛选了 HU 处理后上调并靶向 MITF 基因的 miRNAs。我们发现，HU 处理后 miR-7013-3p 表达增加，并能与 MITF 结合。miR-7013-3p 抑制 B16 黑色素瘤细胞中的黑色素生成、增殖和迁移，并促进细胞凋亡。这些结果可能为 miR-7013-3p 在 B16 黑色素瘤细胞中的作用提供更多信息。
