通过对放射自显影效率进行校正，发现大鼠延髓头端腹外侧区存在高密度的毒蕈碱受体。

A high density of muscarinic receptors in the rostral ventrolateral medulla of the rat is revealed by correction for autoradiographic efficiency.

作者信息

Ernsberger P, Arango V, Reis D J

机构信息

Laboratory of Neurobiology, Cornell University Medical College, New York, NY 10021.

出版信息

Neurosci Lett. 1988 Feb 29;85(2):179-86. doi: 10.1016/0304-3940(88)90348-5.

DOI:10.1016/0304-3940(88)90348-5

PMID:3374834

Abstract

Previous reports of a low density of muscarinic receptors in the vicinity of the rostral ventrolateral reticular nucleus (RVL) conflict with the presence of a muscarinic cholinergic mechanism regulating arterial pressure, and may reflect low autoradiographic efficiency due to quenching of tritium emissions by white matter. Regional autoradiographic efficiency was determined using brains uniformly labeled with O-[3H]methylglucose. Within the RVL, autoradiographic efficiency was only 50 +/- 2% relative to gray matter. The uncorrected density of muscarinic receptors (M2) labeled by [3H]quinuclidinyl benzilate in the RVL appeared to be low relative to cranial nerve nuclei, but the corrected muscarinic receptor density approximated that within the nucleus tractus solitarii. We conclude that a high density of M2 muscarinic acetylcholine receptors is present in the RVL.

摘要

先前有关延髓头端腹外侧网状核（RVL）附近毒蕈碱受体低密度的报道，与存在调节动脉血压的毒蕈碱胆碱能机制相矛盾，并且可能反映了由于白质对氚发射的淬灭导致的放射自显影效率低下。使用用O-[3H]甲基葡萄糖均匀标记的大脑来确定区域放射自显影效率。在RVL内，相对于灰质，放射自显影效率仅为50±2%。用[3H]喹核酯标记的RVL中毒蕈碱受体（M2）的未校正密度相对于颅神经核似乎较低，但校正后的毒蕈碱受体密度接近孤束核内的密度。我们得出结论，RVL中存在高密度的M2毒蕈碱型乙酰胆碱受体。