用于小鼠沙门氏菌病过继性转移治疗的TCRα转导T细胞的生成。

Generation of TCRα-transduced T cells for adoptive transfer therapy of salmonellosis in mice.

作者信息

Kalinina Anastasiia, Bruter Alexandra, Nesterenko Ludmila, Khromykh Ludmila, Kazansky Dmitry

机构信息

Federal State Budgetary Institution "N. N. Blokhin National Medical Research Center of Oncology," the Ministry of Health of the Russian Federation, Moscow 115478, Russia.

Core Facility Centre, Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia.

出版信息

STAR Protoc. 2021 Mar 9;2(1):100368. doi: 10.1016/j.xpro.2021.100368. eCollection 2021 Mar 19.

DOI:10.1016/j.xpro.2021.100368

PMID:33748782

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7972981/

Abstract

Adoptive transfer therapy has great potential to treat diseases such as cancer as well as autoimmune and infectious diseases. Identification of chain-centric T cell receptors (TCRs) with the dominant-active antigen-specific α-chains (TCRα) can significantly improve the efficacy of adoptive cell therapy while reducing time, labor, and costs of generation of TCR-modified antigen-specific T cells. This protocol describes how to generate salmonella-specific TCRα-modified mouse T cells by retroviral transduction and evaluate their functional activity in the mouse model of salmonellosis. For complete details on the use and execution of this protocol, please refer to Kalinina et al. (2020).

摘要

过继性细胞转移疗法在治疗癌症、自身免疫性疾病和感染性疾病等方面具有巨大潜力。鉴定具有显性活性抗原特异性α链（TCRα）的以链为中心的T细胞受体（TCR），可以显著提高过继性细胞疗法的疗效，同时减少生成TCR修饰的抗原特异性T细胞的时间、人力和成本。本方案描述了如何通过逆转录病毒转导生成沙门氏菌特异性TCRα修饰的小鼠T细胞，并在沙门氏菌病小鼠模型中评估其功能活性。有关本方案使用和执行的完整详细信息，请参阅Kalinina等人（2020年）的文献。