Department of Medical Biology, Istanbul School of Medicine, Istanbul University, Istanbul, Turkey.
Department of Pathology, Istanbul School of Medicine, Istanbul University, Istanbul, Turkey.
Transpl Infect Dis. 2021 Aug;23(4):e13605. doi: 10.1111/tid.13605. Epub 2021 Mar 29.
BK virus infections which usually remains asymptomatic in healthy adults may have different clinical manifestations in immunocompromised patient population. BK virus reactivation can cause BK virus nephropathy in 8% of kidney transplant patients and graft loss may be seen if not treated. Clathrin or Caveolar system is known to be required for the transport of many viruses from Polyomaviruses family including BK viruses. In this study, kidney transplant patients with BK virus viremia were divided into two groups according to the BK virus nephropathy found in kidney biopsy (Group I: Viremia+, Nephropathy+ / Group II: Viremia+, Nephropathy-). Kidney biopsies were examined with immunohistochemical staining to determine the distribution and density of the Caveolin-1 and Clathrin molecules. Immunohistochemical staining of the 31 pathologic specimens with anti-caveolin-1 immunoglobulin revealed statistically significant difference between group-I and group-II. The number of the specimens stained with anti-caveolin-1 was less in group I. On the other hand, we did not find any difference between the groups regarding the anti-clathrin immunochemical analysis. According to these findings, caveolin-1 expression differences in kidney transplant patients may be important in disease progression.
BK 病毒感染在健康成年人中通常无症状，但在免疫功能低下的患者中可能有不同的临床表现。BK 病毒再激活可导致 8%的肾移植患者发生 BK 病毒肾病，如果不治疗，可能导致移植物丢失。网格蛋白或小窝蛋白系统已知是包括 BK 病毒在内的多瘤病毒家族中许多病毒从细胞内运输到细胞表面所必需的。在这项研究中，根据肾活检中发现的 BK 病毒肾病，将 BK 病毒血症的肾移植患者分为两组（I 组：病毒血症+，肾病+ / II 组：病毒血症+，肾病-）。用免疫组织化学染色检查肾活检标本，以确定小窝蛋白-1 和网格蛋白分子的分布和密度。用抗小窝蛋白-1 免疫球蛋白对 31 个病理标本进行免疫组织化学染色显示，I 组和 II 组之间存在统计学差异。I 组中染色的标本数量较少。另一方面，我们在抗网格蛋白免疫化学分析方面没有发现两组之间有任何差异。根据这些发现，肾移植患者小窝蛋白-1 表达的差异可能在疾病进展中很重要。
