School of Biomedical Sciences and Pharmacy, University of Newcastle.
School of Biomedical Sciences and Pharmacy, University of Newcastle; Department of Neurology, John Hunter Hospital, Hunter New England Local Health District;
J Vis Exp. 2021 Mar 3(169). doi: 10.3791/62325.
Therapeutic hypothermia (TH) is a powerful neuroprotective strategy that has provided robust evidence for neuroprotection in pre-clinical studies of neurological disorders. Despite strong pre-clinical evidence, TH has not shown efficacy in clinical trials of most neurological disorders. The only successful trials employing therapeutic hypothermia were related to cardiac arrest in adults and hypoxic ischemic injury in neonates. Further investigations into the parameters of its use, and study design comparisons between pre-clinical and clinical studies, are warranted. This article demonstrates two methods of short-duration hypothermia induction. The first method allows for rapid hypothermia induction in rats using ethanol spray and fans. This method works by cooling the skin, which has been less commonly used in clinical trials and may have different physiological effects. Cooling is much more rapid with this technique than is achievable in human patients due to differences in surface area to volume ratio. Along with this, a second method is also presented, which allows for a clinically achievable cooling rate for short-duration hypothermia. This method is easy to implement, reproducible and does not require active skin cooling.
治疗性低温（therapeutic hypothermia，TH）是一种强大的神经保护策略，在神经疾病的临床前研究中提供了强大的神经保护证据。尽管有强有力的临床前证据，但治疗性低温在大多数神经疾病的临床试验中并未显示出疗效。唯一成功的试验是与成人心脏骤停和新生儿缺氧缺血性损伤有关的试验。有必要进一步研究其使用参数，并对临床前和临床研究的研究设计进行比较。本文介绍了两种短时间低温诱导方法。第一种方法使用乙醇喷雾和风扇快速诱导大鼠低温。这种方法通过冷却皮肤来实现，在临床试验中较少使用，可能具有不同的生理效应。由于表面积与体积比的差异，这种技术比在人类患者中实现的冷却速度快得多。与此相关的是，还提出了第二种方法，可实现短时间低温的临床可实现的冷却速度。这种方法易于实施、可重复且不需要主动皮肤冷却。
