College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu, China.
Folia Morphol (Warsz). 2021;80(4):857-869. doi: 10.5603/FM.a2020.0126. Epub 2021 Mar 22.
Heat shock protein 60 (Hsp60) play important roles in protecting testicular development and production of sperms. This study was conducted to investigate Hsp60 gene expression and localisation in testicular development to ascertain its influence on infertility and in different tissues of the male cattle-yak and yak. A total of 54 cattle (24 cattle-yak and 30 yak) were examined.
Heat shock protein 60 mRNA of cattle-yak was cloned first and amino acid variations were found leading to differences at protein spatial structure compare with the yak. Real-time quantitative polymerase chain reaction analysis revealed that Hsp60 mRNAs expression were different in cattle-yak and yak.
The results showed disparity in Hsp60 expression among different tissues and in different developmental stages of the testis. High Hsp60 expression was observed in juvenile and adult testicles. Moreover, Hsp60 expression in cattle-yak was significantly higher than yak (p < 0.01). The location of Hsp60 in tissue and testis was detected by immunohistochemistry and immunofluorescence. The results demonstrated that Hsp60 proteins located in epithelial cells, spermatocytes, sperm cells and mesenchymal cells.
The Hsp60 proteins are expressed in different tissues, and the highest expression level was observed in the testis of the cattle-yak, which suggests that infertility of cattle-yak have some correlation with up-regulation of Hsp60.
热休克蛋白 60（Hsp60）在保护睾丸发育和精子生成中发挥重要作用。本研究旨在探讨 Hsp60 基因在睾丸发育过程中的表达和定位，以确定其对不育症的影响，并研究其在雄性牦牛和牦牛不同组织中的表达。共检查了 54 头牛（24 头牦牛和 30 头牦牛）。
首先克隆了牦牛的热休克蛋白 60 mRNA，发现其氨基酸变异导致与牦牛相比蛋白质空间结构存在差异。实时定量聚合酶链反应分析显示，Hsp60 mRNA 在牦牛和牦牛中的表达存在差异。
结果表明，Hsp60 在不同组织和睾丸发育阶段的表达存在差异。幼体和成年睾丸中 Hsp60 的表达较高。此外，牦牛的 Hsp60 表达明显高于牦牛（p < 0.01）。通过免疫组织化学和免疫荧光检测组织和睾丸中 Hsp60 的定位。结果表明，Hsp60 蛋白定位于上皮细胞、精母细胞、精子细胞和间质细胞。
Hsp60 蛋白在不同组织中表达，在牦牛睾丸中的表达水平最高，这表明牦牛不育与 Hsp60 的上调有关。
