A lysosome-targeted probe for the real-time detection of hypobromous acid in living human cancer cells.

We reported here a naphthalimide-based fluorescent probe LysOBr that localizes in the lysosome in live cells. LysOBr exhibits excellent HOBr selectivity and desirable optical properties. It can quantitatively detect lysosomal HOBr at 0-20 μM, with a detection limit of 243 nM. The short (4 s) response time allows real-time HOBr detection and imaging, as shown with studies in live HeLa cancer cells. It is thus the most rapidly responsive HOBr probe to date, among the most selective ones, and the first probe that is lysosome-specific with a "turn-on" signal. The probe structure is modular, and convenient structural modification should lead to other organelle-specific probes.