大鼠视网膜、松果体和哈德氏腺中抗利尿激素和催产素的昼夜节律。

A day/night rhythm of vasopressin and oxytocin in rat retina, pineal and harderian gland.

作者信息

Gauquelin G, Gharib C, Ghaemmaghami F, Allevard A M, Cherbal F, Geelen G, Bouzeghrane F, Legros J J

机构信息

Laboratoire de Physiologie, Faculté de Médecine Grange-Blanche, Lyon, France.

出版信息

Peptides. 1988 Mar-Apr;9(2):289-93. doi: 10.1016/0196-9781(88)90262-8.

DOI:10.1016/0196-9781(88)90262-8

PMID:3375136

Abstract

Arginine vasopressin (AVP), oxytocin (OT) and neurophysins (Np) have been found in the pineal gland and the retina of the rat. Because the retina, pineal gland and Harderian gland (HG) serve analogous functions, we undertook a study to determine the presence of these peptides in these three organs of rats. They were detected by two specific methods: HPLC and specific radioimmunoassays. For Np, total neurophysins (NpT) were measured. To determine a 24 hr rhythm, the animals were maintained under a light/dark cycle of 12 hr/12 hr for 3 weeks. The pineal glands, retinae and HG were collected. Day/night rhythms of AVP, OT and NpT were demonstrated in the retina and HG; but the pineal gland had only AVP rhythm. A significant decrease in the rhythms at 4 a.m. was demonstrated in the retina and HG. The 24 hr variation of AVP in the retina seemed parallel to that of the HG.

摘要

在大鼠的松果体和视网膜中发现了精氨酸加压素（AVP）、催产素（OT）和神经垂体素（Np）。由于视网膜、松果体和哈德氏腺（HG）具有类似的功能，我们开展了一项研究来确定这些肽在大鼠这三个器官中的存在情况。通过两种特定方法对它们进行检测：高效液相色谱法（HPLC）和特异性放射免疫测定法。对于Np，测定了总神经垂体素（NpT）。为了确定24小时节律，将动物置于12小时光照/12小时黑暗的周期下饲养3周。收集松果体、视网膜和HG。在视网膜和HG中证实了AVP、OT和NpT的昼夜节律；但松果体仅有AVP节律。在凌晨4点时，视网膜和HG中的节律显著下降。视网膜中AVP的24小时变化似乎与HG的变化平行。