人胃分泌物中的免疫提取降钙素。

Immunoextracted calcitonin in human gastric secretion.

作者信息

Bucht E, Wisén O, Granberg B, Sjöberg H E

机构信息

Department of Endocrinology, Karolinska Institute and Hospital, Stockholm, Sweden.

出版信息

Peptides. 1988 Mar-Apr;9(2):295-9. doi: 10.1016/0196-9781(88)90263-x.

DOI:10.1016/0196-9781(88)90263-x

PMID:3375137

Abstract

Immunoreactive calcitonin (iCT) has been demonstrated in human gastric juice after immunoextraction with immobilized antibodies and subsequent radioimmunoassay. The basal levels were 4.5 +/- 3.1 (mean +/- SD) pg-Eq/ml gastric juice; range 1.2-9.1 pg-Eq/ml; n = 7, and after stimulatory gastric secretion test with pentagastrin 0.3 +/- 0.2 pg-Eq/ml; range 0.1-0.7 pg-Eq/ml; n = 7 (p less than 0.01). The main fraction of iCT from gastric juice eluted in the same region as synthetic human calcitonin (hCT) on Sephadex G-75 gel chromatography. Reverse phase chromatography in a fast protein liquid chromatography (FPLC) system revealed a slightly less hydrophobic character of the iCT from gastric juice compared to synthetic monomeric hCT. The results were further confirmed by using an additional antiserum. In plasma, the calcitonin (CT) levels were after immunoextraction at the basal state 6.6 +/- 1.7 pg-Eq/ml (mean +/- SD); range 5.1-10.1 pg-Eq/ml; n = 7 and after pentagastrin stimulation 9.4 +/- 5.4 pg-Eq/ml; range 6.3-18.5 pg-Eq/ml; n = 7.

摘要

用固定化抗体进行免疫提取并随后进行放射免疫测定后，已在人胃液中证实存在免疫反应性降钙素（iCT）。基础水平为4.5±3.1（平均值±标准差）pg-Eq/ml胃液；范围为1.2 - 9.1 pg-Eq/ml；n = 7，在使用五肽胃泌素进行刺激性胃分泌试验后为0.3±0.2 pg-Eq/ml；范围为0.1 - 0.7 pg-Eq/ml；n = 7（p < 0.01）。在Sephadex G - 75凝胶色谱上，胃液中iCT的主要部分与合成人降钙素（hCT）在同一区域洗脱。快速蛋白质液相色谱（FPLC）系统中的反相色谱显示，与合成单体hCT相比，胃液中iCT的疏水性略低。使用另一种抗血清进一步证实了结果。在血浆中，基础状态下免疫提取后的降钙素（CT）水平为6.6±1.7 pg-Eq/ml（平均值±标准差）；范围为5.1 - 10.1 pg-Eq/ml；n = 7，五肽胃泌素刺激后为9.4±5.4 pg-Eq/ml；范围为6.3 - 18.5 pg-Eq/ml；n = 7。