甲醇营养酵母 Ogataea polymorpha 中的醇氧化酶：分离、纯化和生物分析应用。

Alcohol Oxidase from the Methylotrophic Yeast Ogataea polymorpha: Isolation, Purification, and Bioanalytical Application.

机构信息

Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine.

Drohobych Ivan Franko State Pedagogical University, Drohobych, Ukraine.

出版信息

Methods Mol Biol. 2021;2280:231-248. doi: 10.1007/978-1-0716-1286-6_15.

DOI:10.1007/978-1-0716-1286-6_15

PMID:33751439

Abstract

Alcohol oxidase (EC 1.1.3.13; AOX) is a flavoprotein that catalyzes the oxidation of primary short-chain alcohols to corresponding carbonyl compounds with a concomitant release of hydrogen peroxide. It is a key enzyme of methanol metabolism in methylotrophic yeasts, catalyzing the first step of methanol oxidation to formaldehyde.Here we describe the isolation and purification of AOX from the thermotolerant methylotrophic yeast Ogataea (Hansenula) polymorpha, and using this enzyme in enzymatic assay of ethanol, simultaneous analysis of methanol and formaldehyde, and in construction of amperometric biosensors selective to primary alcohols and formaldehyde.

摘要

醇氧化酶（EC 1.1.3.13；AOX）是一种黄素蛋白，能够催化伯短链醇氧化为相应的羰基化合物，同时释放过氧化氢。它是甲醇营养型酵母甲醇代谢的关键酶，催化甲醇氧化为甲醛的第一步。本文描述了耐热甲醇营养型酵母 Ogataea（Hansenula）polymorpha 中 AOX 的分离和纯化，并利用该酶进行乙醇酶促分析、甲醇和甲醛的同时分析，以及构建对伯醇和甲醛具有选择性的电流型生物传感器。