Exosomal miR-122-5p inhibits tumorigenicity of gastric cancer by downregulating .

BACKGROUND

microRNAs (miRNAs) are non-coding RNAs with important roles in the progression of human cancers, including gastric cancer. Exosomes are extracellular vesicles, which could transfer numerous noncoding RNAs, such as miRNAs. Here, in our study, we intended to investigate the role of exosomal miR-122-5p in gastric cancer progression.

METHODS

Exosomes were isolated utilizing commercial kit or ultracentrifugation. Biomarkers of exosomes or epithelia-mesenchymal transition (EMT) were monitored by western blot. Expression levels of miR-122-5p and G-protein-coupled receptor kinase interacting protein-1 () were evaluated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) or western blot. Cell proliferation and apoptosis were assessed by colony formation assay, methyl thiazolyl tetrazolium assay and flow cytometry. Cell metastasis was evaluated via Transwell assay. The interaction between miR-122-5p and was validated by dual-luciferase reporter assay. Furthermore, tumor growth in vivo was detected by xenograft assay.

RESULTS

Exosomes were successfully isolated. MiR-122-5p was downregulated in exosomes derived from the serum of gastric cancer patients. Exosomal miR-122-5p could hinder gastric cancer cell proliferation and metastasis in vitro and tumor growth in vivo. Knockdown of also inhibited gastric cancer cell proliferation and metastasis. Exosomal miR-122-5p targeted to alter cellular behaviors of gastric cancer cells.

CONCLUSION

Exosomal miR-122-5p suppressed gastric cancer progression by targeting .