CXCL5在宫腔粘连发病机制中的表达及潜在作用
Expression and potential role of CXCL5 in the pathogenesis of intrauterine adhesions.
作者信息
Fang Zi-Ang, He Yu, Sun Chao, Zhan Lei, Zhou Guiju, Wei Bing, Sun Shiying
机构信息
Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Anhui Medical University, Hefei, China.
出版信息
J Int Med Res. 2021 Mar;49(3):300060521997718. doi: 10.1177/0300060521997718.
OBJECTIVE
C-X-C motif chemokine ligand 5 (CXCL5), a member of the chemokine family, is associated with remodeling of connective tissues. However, its role in formation of intrauterine adhesions (IUA) remains unclear. We aimed to investigate the expression and mechanism underlying the role of CXCL5 in IUA.
METHODS
Expression of CXCL5 in IUA was detected by immunohistochemistry in a rat model of IUA and by real-time PCR and western blotting in patients with IUA. The protein levels of matrix metalloproteinase 9 (MMP9) and transcription factor p65 in human endometrial cells were assessed by western blotting after CXCL5 overexpression.
RESULTS
Protein expression of CXCL5 was significantly decreased in the endometria of IUA rats compared with that of control and sham-operated rats. Real-time PCR and western blotting in patients with IUA showed similar results to those from the rat model. After overexpression, CXCL5 significantly upregulated expression of MMP9 and slightly upregulated expression of p65 in human endometrial cells.
CONCLUSIONS
CXCL5 plays an important role in IUA formation after endometrial injury. We propose a molecular mechanism to explain formation of IUA, including downregulation of MMP9 by low CXCL5 expression. These findings provide valuable information for the prevention and targeted therapy of IUA.
目的
C-X-C基序趋化因子配体5（CXCL5）是趋化因子家族的一员，与结缔组织重塑相关。然而，其在宫腔粘连（IUA）形成中的作用仍不清楚。我们旨在研究CXCL5在IUA中的表达及其作用机制。
方法
通过免疫组织化学在IUA大鼠模型中检测CXCL5的表达，并通过实时PCR和蛋白质印迹法在IUA患者中检测。CXCL5过表达后，通过蛋白质印迹法评估人子宫内膜细胞中基质金属蛋白酶9（MMP9）和转录因子p65的蛋白水平。
结果
与对照大鼠和假手术大鼠相比，IUA大鼠子宫内膜中CXCL5的蛋白表达显著降低。IUA患者的实时PCR和蛋白质印迹结果与大鼠模型相似。过表达后，CXCL5显著上调人子宫内膜细胞中MMP9的表达，并轻微上调p65的表达。
结论
CXCL5在子宫内膜损伤后的IUA形成中起重要作用。我们提出了一种分子机制来解释IUA的形成，包括低CXCL5表达导致MMP9下调。这些发现为IUA的预防和靶向治疗提供了有价值的信息。
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