苯妥英钠和皮质醇对细胞周期的影响。

The effect of diphenylhydantoin and cortisol on the cell cycle.

作者信息

MacKinney A A, Knobeloch L, Norback D

机构信息

Hematology Laboratory, William S. Middleton Memorial Veterans Hospital, Madison, Wisconsin 53705.

出版信息

Proc Soc Exp Biol Med. 1988 Jun;188(2):173-6. doi: 10.3181/00379727-188-42724.

DOI:10.3181/00379727-188-42724

PMID:3375264

Abstract

Normal human lymphocytes cultured in the presence of phytohemagglutinin were blocked in G0G1 when diphenylhydantoin (DPH) or cortisol 3.6 X 10(-4) M was added at the beginning of culture. The suppression of culture growth was analyzed by flow cytometry and confirmed by [3H]thymidine incorporation and mitotic rate analysis. The correlation of these measurements with flow cytometry was good for DNA synthesis and excellent for mitosis. There was an additive effect on the G0G1 retention of cells when both drugs were present in the culture. These data may partially explain the suppression of cell-mediated immunity which occurs in DPH-treated patients.

摘要

在含有植物血凝素的条件下培养的正常人淋巴细胞，当在培养开始时加入二苯乙内酰脲（DPH）或3.6×10⁻⁴M皮质醇时，会被阻滞在G0G1期。通过流式细胞术分析培养生长的抑制情况，并通过[³H]胸腺嘧啶核苷掺入和有丝分裂率分析加以证实。这些测量值与流式细胞术在DNA合成方面相关性良好，在有丝分裂方面相关性极佳。当两种药物同时存在于培养物中时，对细胞在G0G1期的滞留具有相加作用。这些数据可能部分解释了在接受DPH治疗的患者中发生的细胞介导免疫抑制现象。