作为诊断献血者缺铁的标志物的铁调素。

Hepcidin as a diagnostic marker of iron deficiency in blood donors.

机构信息

Department of Transfusion Medicine, All India Institute of Medical Sciences, Rishikesh, 249203, India.

Department of Transfusion Medicine, Government Medical College and Hospital, Chandigarh, 160030, India.

出版信息

Transfus Apher Sci. 2021 Jun;60(3):103121. doi: 10.1016/j.transci.2021.103121. Epub 2021 Mar 13.

DOI:10.1016/j.transci.2021.103121

PMID:33752992

Abstract

BACKGROUND

Blood donors are prone to have iron deficiency. The aim of this study was to determine utility of serum hepcidin as an indicator of iron deficiency in blood donors.

METHODS

A total of 200 voluntary, healthy blood donors were included in the study. Donors were categorized into four groups according to the donation frequency. Group I: (n = 50) who donated for the first time, or those who have not donated in the past 2 years (reactivated donors). Group II: (n = 50), who donated blood for the second time and had donated once in the previous 12 months. Group III: (n = 50), who donated blood for third time and had donated twice in the previous 12 months. Group IV: (n = 50) who donated blood for the fourth time and had donated thrice in the previous 12 months. Sera of study participants were evaluated for serum ferritin and serum hepcidin levels based on enzyme linked immunosorbent assay.

RESULTS

Serum hepcidin concentration ranged 2.36-15734 pg/mL. Serum hepcidin and serum ferritin were found to be lowest in group IV donors. When serum ferritin concentration of less than 15 ng/mL was considered as gold standard for diagnosing iron deficiency, AUCROC for serum hepcidin as a diagnostic test of iron deficiency was found to be 0.715. Serum hepcidin showed statistical significant correlation with donation frequency(p = 0 .005) and serum ferritin (p = 0.01). Sensitivity and specificity of serum hepcidin was found to be 77.8 %, 79.6 % respectively.

CONCLUSION

Effectiveness of Serum hepcidin as a diagnostic marker of iron deficiency still needs to be determined.

摘要

背景

献血者容易缺铁。本研究旨在确定血清hepcidin 作为献血者缺铁指标的效用。

方法

共纳入 200 名自愿、健康的献血者进行研究。根据献血频率将献血者分为四组。I 组（n = 50）：首次献血者或过去 2 年未献血者（再激活献血者）。II 组（n = 50）：第二次献血者，且在过去 12 个月内曾献血一次。III 组（n = 50）：第三次献血者，且在过去 12 个月内曾献血两次。IV 组（n = 50）：第四次献血者，且在过去 12 个月内曾献血三次。通过酶联免疫吸附试验评估研究参与者的血清铁蛋白和血清 hepcidin 水平。

结果

血清 hepcidin 浓度范围为 2.36-15734 pg/mL。发现 IV 组献血者的血清 hepcidin 和血清铁蛋白最低。当血清铁蛋白浓度<15ng/mL 被认为是诊断缺铁的金标准时，血清 hepcidin 作为缺铁诊断试验的 AUCROC 为 0.715。血清 hepcidin 与献血频率呈统计学显著相关（p = 0.005）和血清铁蛋白（p = 0.01）。血清 hepcidin 的敏感性和特异性分别为 77.8%和 79.6%。