Department of Nuclear Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
Department of Radiology, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
Hepatobiliary Pancreat Dis Int. 2021 Aug;20(4):345-351. doi: 10.1016/j.hbpd.2021.03.001. Epub 2021 Mar 9.
Positron emission tomography (PET) imaging is a non-invasive method to visualize and quantify the tumor microenvironment. This study aimed to explore the feasibility of F-AIF-NOTA-E[PEG-c(RGDfk)] (denoted as F-RGD) PET quantitative parameters to distinguish the angiogenesis in colorectal cancer (CRC) mice which has different metastatic potential.
Twenty LoVo and twenty LS174T of CRC liver metastases animal models were established by implantation of human CRC cell lines via intrasplenic injection. Radiotracer-based micro-PET imaging of animal model was performed and the uptake of F-RGD tracer in the tumor tissues was quantified as tumor-to-liver maximum or mean standardized uptake value (SUVmax or SUVmean) ratio. Pearson correlation was used to analyze the relationship between radioactive parameters and tumor markers.
The SUVmax and SUVmean ratios of LoVo model were significantly higher than those of LS174T in both liver metastasis and primary tumor lesions (P < 0.05). A significant difference was observed in both vascular endothelial growth factor (VEGF) and Ki67 expressions between LoVo and LS174T primary tumors (P < 0.05). The tumor-to-liver SUVmax or SUVmean ratio of F-RGD showed a moderate correlation with VEGF expression (r = 0.5700, P = 0.001 and r = 0.6657, P < 0.001, respectively), but the SUVmean ration showed a weak correlation with Ki67 expression (r = 0.3706, P < 0.05). The areas under the receiver operating characteristic (ROC) curves of F-RGD SUVmean ratio, SUVmax ratio for differentiating LoVo from LS174T tumor were 0.801 and 0.759, respectively.
The tumor-to-liver SUVmean ratio of F-RGD was a promising image parameter for the process of monitoring tumor angiogenesis in CRC xenograft mice model.
正电子发射断层扫描（PET）成像技术是一种非侵入性的方法，可以可视化和量化肿瘤微环境。本研究旨在探索 F-AIF-NOTA-E[PEG-c（RGDfk）]（表示为 F-RGD）的 PET 定量参数的可行性，以区分具有不同转移潜能的结直肠癌（CRC）小鼠的血管生成。
通过脾脏内注射人 CRC 细胞系，建立 20 例 LoVo 和 20 例 LS174T 的 CRC 肝转移动物模型。对动物模型进行基于放射性示踪剂的 micro-PET 成像，并对肿瘤组织中 F-RGD 示踪剂的摄取进行定量，即肿瘤与肝脏的最大或平均标准化摄取值（SUVmax 或 SUVmean）比值。采用 Pearson 相关性分析放射性参数与肿瘤标志物之间的关系。
LoVo 模型的肝转移和原发肿瘤病变的 SUVmax 和 SUVmean 比值均显著高于 LS174T（P < 0.05）。LoVo 和 LS174T 原发肿瘤的血管内皮生长因子（VEGF）和 Ki67 表达均存在显著差异（P < 0.05）。F-RGD 的肿瘤与肝脏 SUVmax 或 SUVmean 比值与 VEGF 表达呈中度相关（r=0.5700，P=0.001 和 r=0.6657，P<0.001），但 SUVmean 比值与 Ki67 表达呈弱相关（r=0.3706，P<0.05）。F-RGD SUVmean 比值、SUVmax 比值区分 LoVo 和 LS174T 肿瘤的受试者工作特征（ROC）曲线下面积分别为 0.801 和 0.759。
F-RGD 的肿瘤与肝脏 SUVmean 比值是监测 CRC 异种移植小鼠模型肿瘤血管生成过程的有前途的影像参数。
