Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, China.
Hunan Key Laboratory of Ophthalmology, Changsha, Hunan, China.
Wiley Interdiscip Rev RNA. 2021 Sep;12(5):e1652. doi: 10.1002/wrna.1652. Epub 2021 Mar 22.
Single-cell RNA sequencing (scRNA-seq) is a technology for single-cell transcriptome analysis that can be used to characterize complex dynamics of various retinal cell types. It provides deep scrutiny into the gene expression character of diverse cell types, lending insight into all the biological processes being carried out. The scRNA-seq is an alternative to regular RNA-seq, which does not achieve cellular heterogeneity. The retina, is a part of the central nervous system (CNS) and consists of six types of neurons and several types of glial cells. Studying retinal cell heterogeneity is important for understanding retinal diseases. Currently, scRNA-seq is employed to assess retina development and retinal disease pathogenesis and has improved our understanding of the relationship between the retina, its visual pathways, and the brain. Moreover, this technology provides new ideas on the sensitivity and molecular mechanisms of cell subtypes involved in retinal-related diseases. The application of scRNA-seq technology has given us a deeper understanding of the latest advancements and challenges in retinal development and diseases. We advocate scRNA-seq as one of the important tools for developing novel therapies for retinal diseases. This article is categorized under: RNA Methods > RNA Analyses in Cells RNA in Disease and Development > RNA in Development RNA in Disease and Development > RNA in Disease.
单细胞 RNA 测序（scRNA-seq）是一种单细胞转录组分析技术，可用于描述各种视网膜细胞类型的复杂动态。它深入研究了不同细胞类型的基因表达特征，洞察了所有正在进行的生物学过程。scRNA-seq 是常规 RNA-seq 的一种替代方法，后者无法实现细胞异质性。视网膜是中枢神经系统（CNS）的一部分，由六种神经元和几种神经胶质细胞组成。研究视网膜细胞异质性对于理解视网膜疾病很重要。目前，scRNA-seq 用于评估视网膜发育和视网膜疾病发病机制，并提高了我们对视网膜、其视觉通路和大脑之间关系的理解。此外，这项技术为涉及视网膜相关疾病的细胞亚型的敏感性和分子机制提供了新的思路。scRNA-seq 技术的应用使我们更深入地了解了视网膜发育和疾病的最新进展和挑战。我们提倡将 scRNA-seq 作为开发视网膜疾病新疗法的重要工具之一。本文归类于：RNA 方法 > RNA 在细胞中的分析 RNA 在疾病与发育中 > RNA 在发育中 RNA 在疾病与发育中 > RNA 在疾病中。
