College of Forestry, Shanxi Agricultural University, Jinzhong 030801, Shanxi, China.
Ying Yong Sheng Tai Xue Bao. 2021 Mar;32(3):853-859. doi: 10.13287/j.1001-9332.202103.004.
Tree regeneration is significantly affected by water deficiency, which could be alleviated by the inoculation of spp In this study, the effects of rhizosphere inoculation with spp on the antioxidant system and osmotic substances in the seedlings of was investigated under experimental drought stresses, with the relative water content 50%-60%, 35%-50%, and 20%-35% as the light, moderate and severe drought stress, respectively. The results showed that, with increasing drought stress, the activities of protective enzymes, SOD and POD in the needles of seedlings were significantly promoted, the contents of MDA and proline were remarkably increased, whereas the contents of soluble protein and starch showed a decreasing trend. Inoculation with spp promoted much stronger increase in the activities of SOD and POD in the needles, yet the MDA content showed a lower level of increase. Under moderate drought, the activity of POD in the seedling inoculated with spp was 1.8 folds of that in the control, while the content of MDA was 62.9% of the control. The contents of proline, soluble protein, soluble sugar, and starch in the inoculated seedlings were higher than that in the uninoculated seedlings. Drought-resistance of seedlings inoculated by spp. was significantly enhanced due to the augmenting regulation of antioxidant system and osmotic adjustment substances. Therefore, under the trend of warming and drying in North China, the technique of rhizosphere inoculation of spp. could be popularized for forest plantation or understory tending.
树木再生受到水分缺乏的显著影响，而 spp 的接种可以缓解这种影响。本研究在实验干旱胁迫下，以相对水分为 50%-60%、35%-50%和 20%-35%的轻度、中度和重度干旱胁迫为例，调查了 spp 根际接种对 幼苗抗氧化系统和渗透物质的影响。结果表明，随着干旱胁迫的增加，幼苗针叶中保护酶 SOD 和 POD 的活性显著提高，MDA 和脯氨酸的含量显著增加，而可溶性蛋白和淀粉的含量呈下降趋势。接种 spp 可显著促进 SOD 和 POD 在针叶中的活性增加，但 MDA 含量的增加幅度较低。在中度干旱下，接种 spp 的幼苗中 POD 的活性是对照的 1.8 倍，而 MDA 的含量是对照的 62.9%。接种幼苗中脯氨酸、可溶性蛋白、可溶性糖和淀粉的含量均高于未接种的幼苗。由于抗氧化系统和渗透调节物质的增强调节，接种 spp 的 幼苗的耐旱性显著增强。因此，在中国北方气候变暖干燥的趋势下，可以推广 spp 的根际接种技术，用于森林种植或林下抚育。
