Department of Orthopedic Oncology Surgery, Shandong Cancer Hospital, 66555Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.
Department of Orthopaedics, Shandong Provincial Third Hospital, 66555Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.
Technol Cancer Res Treat. 2021 Jan-Dec;20:15330338211004918. doi: 10.1177/15330338211004918.
Osteosarcoma often occurs in children and adolescents and causes poor prognosis. The role of RNA-binding proteins (RBPs) in malignant tumors has been elucidated in recent years. Our study aims to identify key RBPs in osteosarcoma that could be prognostic factors and treatment targets. GSE33382 dataset was downloaded from Gene Expression Omnibus (GEO) database. RBPs extraction and differential expression analysis was performed. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed to explore the biological function of differential expression RBPs. Moreover, we constructed Protein-protein interaction (PPI) network and obtained key modules. Key RBPs were identified by univariate Cox regression analysis and multiple stepwise Cox regression analysis combined with the clinical information from Therapeutically Applicable Research to Generate Effective Treatments (TARGET) database. Risk score model was generated and validated by GSE16091 dataset. A total of 38 differential expression RBPs was identified. Go and KEGG results indicated these RBPs were significantly involved in ribosome biogenesis and mRNA surveillance pathway. COX regression analysis showed DDX24, DDX21, WARS and IGF2BP2 could be prognostic factors in osteosarcoma. Spearman's correlation analysis suggested that WARS might be important in osteosarcoma immune infiltration. In conclusion, DDX24, DDX21, WARS and IGF2BP2 might play key role in osteosarcoma, which could be therapuetic targets for osteosarcoma treatment.
骨肉瘤常发生于儿童和青少年，预后较差。近年来，RNA 结合蛋白（RBPs）在恶性肿瘤中的作用已被阐明。我们的研究旨在鉴定骨肉瘤中可能作为预后因素和治疗靶点的关键 RBPs。从基因表达综合数据库（GEO）下载 GSE33382 数据集。进行 RBPs 提取和差异表达分析。进行基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集分析，以探讨差异表达 RBPs 的生物学功能。此外，我们构建了蛋白质-蛋白质相互作用（PPI）网络，并获得了关键模块。通过单变量 Cox 回归分析和结合 Therapeutically Applicable Research to Generate Effective Treatments（TARGET）数据库中的临床信息的多步 Cox 回归分析来鉴定关键 RBPs。使用 GSE16091 数据集生成和验证风险评分模型。鉴定出 38 个差异表达的 RBPs。GO 和 KEGG 结果表明这些 RBPs 显著参与核糖体生物发生和 mRNA 监测途径。COX 回归分析显示 DDX24、DDX21、WARS 和 IGF2BP2 可能是骨肉瘤的预后因素。Spearman 相关性分析表明 WARS 可能在骨肉瘤免疫浸润中起重要作用。总之，DDX24、DDX21、WARS 和 IGF2BP2 可能在骨肉瘤中发挥关键作用，可能成为骨肉瘤治疗的治疗靶点。
