Food Toxicology Laboratory, School of Food Science and Technology, Chung-Ang University, Anseong 17546, South Korea.
Food Toxicology Laboratory, School of Food Science and Technology, Chung-Ang University, Anseong 17546, South Korea.
J Hazard Mater. 2021 Aug 5;415:125663. doi: 10.1016/j.jhazmat.2021.125663. Epub 2021 Mar 15.
Aflatoxins are a group of carcinogenic and mutagenic fungal secondary metabolites that have threatened human health and global food security. Aflatoxin contamination can be controlled by applying fungicides, such as strobilurins. Although these compounds have been effective, they may be risky to the environment due to their wide usage. In this study, plant-based compounds were tested to promote the performance of strobilurins (azoxystrobin, pyraclostrobin) against aflatoxigenic Aspergillus flavus; six natural compounds, namely baicalein, nobiletin, meso-dihydroguaiaretic acid, pinoresinol, syringaresinol, and celastrol, were found to exhibit synergistic antifungal effects with strobilurins with fractional inhibitory concentration index < 0.5. Among them, baicalein showed no inhibitory effects on A. flavus when applied alone, but strongly enhanced the in vitro and in situ antifungal and antiaflatoxigenic efficacy of strobilurins and transformed them from fungistatic to fungicidal agents. Therefore, baicalein may be used as an effective natural chemosensitizing agent to improve the performance of strobilurins against A. flavus. The findings of this study provide novel insights for the development of safer and more effective strategies for the control of aflatoxin contamination.
黄曲霉毒素是一类具有致癌性和致突变性的真菌次级代谢产物，威胁着人类健康和全球食品安全。可以通过使用杀菌剂（如 strobilurins）来控制黄曲霉毒素污染。虽然这些化合物已经很有效，但由于它们的广泛使用，可能对环境有风险。在这项研究中，测试了植物源化合物，以提高 strobilurins（肟菌酯、吡唑醚菌酯）对产黄曲霉毒素的 Aspergillus flavus 的性能；发现六种天然化合物，即黄芩素、诺必灵、meso-二氢愈创木酸、松脂醇、丁香脂素和雷公藤红素，与 strobilurins 具有协同抗真菌作用，其分数抑制浓度指数 < 0.5。其中，黄芩素单独使用时对 A. flavus 没有抑制作用，但能强烈增强 strobilurins 的体外和原位抗真菌和抗黄曲霉毒素功效，并将其从抑菌剂转变为杀菌剂。因此，黄芩素可用作有效的天然化学增敏剂，以提高 strobilurins 对 A. flavus 的性能。这项研究的结果为开发更安全、更有效的控制黄曲霉毒素污染的策略提供了新的见解。
