基于金纳米粒子与 InP/ZnS 量子点之间 FRET 的灵敏免疫传感器用于精氨酸激酶检测。

A sensitive immunosensor based on FRET between gold nanoparticles and InP/ZnS quantum dots for arginine kinase detection.

机构信息

Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.

出版信息

Food Chem. 2021 Aug 30;354:129536. doi: 10.1016/j.foodchem.2021.129536. Epub 2021 Mar 11.

DOI:10.1016/j.foodchem.2021.129536

PMID:33756326

Abstract

Arginine kinase (AK) is one of the most important allergens in shrimp products. Herein, a novel immunoassay for quantitation of AK was developed using the antibody modified gold nanoparticle (AuNP) and quantum dot (QD). When the first antibody modified AuNP (AuNP-Ab1) was bridged by AK with the secondary antibody modified QD (QD-Ab2), fluorescence resonance energy transfer (FRET) would occur between the AuNP and QD, which led to a decrease in fluorescent signals. The decrease in fluorescence intensity was found to correlate linearly with the log of AK concentration in the range of 1.0 × 10-1.0 × 10 mg/mL (R = 0.9909) and the detection limit was 0.11 ng/mL. The immunoassay was further proved to have encouraging specificity, precision and accuracy. Compared with existing methods, this study provided a promising approach to develop a highly sensitive and selective detection method for AK in shrimp related food samples.

摘要

精氨酸激酶（AK）是虾类产品中最重要的过敏原之一。本文利用抗体修饰的金纳米粒子（AuNP）和量子点（QD）建立了一种用于定量检测 AK 的新型免疫分析方法。当第一抗体修饰的 AuNP（AuNP-Ab1）被 AK 桥接，与第二抗体修饰的 QD（QD-Ab2）结合时，AuNP 和 QD 之间会发生荧光共振能量转移（FRET），导致荧光信号减弱。荧光强度的降低与 AK 浓度的对数在 1.0×10-1.0×10 mg/mL 范围内呈线性相关（R=0.9909），检测限为 0.11 ng/mL。该免疫分析方法进一步证明具有令人鼓舞的特异性、精密度和准确性。与现有方法相比，本研究为开发虾类相关食品中 AK 的高灵敏度和选择性检测方法提供了一种很有前景的方法。