William Nicola, Bamidoro Faith, Beales Paul A, Drummond-Brydson Rik, Hondow Nicole, Key Sarah, Kulak Alexander, Walsh Aidan Charles, Winter Sophia, Nelson Laurence Andrew
School of Chemistry, University of Leeds, Leeds LS2 9JT, UK.
School of Chemical and Process Engineering, University of Leeds, Leeds LS2 9JT, UK.
J Colloid Interface Sci. 2021 Jul 15;594:101-112. doi: 10.1016/j.jcis.2021.03.009. Epub 2021 Mar 10.
The properties of stable gold (Au) nanoparticle dispersions can be tuned to alter their activity towards biomembrane models.
Au nanoparticle coating techniques together with rapid electrochemical screens of a phospholipid layer on fabricated mercury (Hg) on platinum (Pt) electrode have been used to moderate the phospholipid layer activity of Au nanoparticle dispersions. Screening results for Au nanoparticle dispersions were intercalibrated with phospholipid large unilamellar vesicle (LUV) interactions using a carboxyfluorescein (CF) leakage assay. All nanoparticle dispersions were characterised for size, by dynamic light scattering (DLS) and transmission electron microscopy (TEM).
Commercial and high quality home synthesised Au nanoparticle dispersions are phospholipid monolayer active whereas Ag nanoparticle dispersions are not. If Au nanoparticles are coated with a thin layer of Ag then the particle/lipid interaction is suppressed. The electrochemical assays of the lipid layer activity of Au nanoparticle dispersions align with LUV leakage assays of the same. Au nanoparticles of decreasing size and increasing dispersion concentration showed a stronger phospholipid monolayer/bilayer interaction. Treating Au nanoparticles with cell culture medium and incubation of Au nanoparticle dispersions in phosphate buffered saline (PBS) solutions removes their phospholipid layer interaction.
可以调节稳定的金（Au）纳米颗粒分散体的性质，以改变其对生物膜模型的活性。
金纳米颗粒涂层技术与在铂（Pt）电极上制备的汞（Hg）上的磷脂层的快速电化学筛选一起，用于调节金纳米颗粒分散体的磷脂层活性。使用羧基荧光素（CF）泄漏测定法，将金纳米颗粒分散体的筛选结果与磷脂大单层囊泡（LUV）相互作用进行相互校准。通过动态光散射（DLS）和透射电子显微镜（TEM）对所有纳米颗粒分散体的尺寸进行了表征。
商业和高质量的自制金纳米颗粒分散体具有磷脂单层活性，而银纳米颗粒分散体则没有。如果金纳米颗粒涂覆有一层薄银，则颗粒/脂质相互作用会受到抑制。金纳米颗粒分散体的脂质层活性的电化学测定与相同的LUV泄漏测定结果一致。尺寸减小且分散浓度增加的金纳米颗粒表现出更强的磷脂单层/双层相互作用。用细胞培养基处理金纳米颗粒并将金纳米颗粒分散体在磷酸盐缓冲盐水（PBS）溶液中孵育会消除它们的磷脂层相互作用。
