Rigottier-Gois Lionel, Turner Sarah L, Young J Peter W, Amarger Noëlle
Laboratoire de Microbiologie des Sols, Institut National de la Recherche Agronomique,17 rue Sully, BV 1540, 21034 Dijon cedex,France.
Department of Biology, University of York,PO Box 373, York YO1 5YW,UK.
Microbiology (Reading). 1998 Mar;144(3):771-780. doi: 10.1099/00221287-144-3-771.
The distribution of four classes of related plasmid replication genes () within three field populations of in France, Germany and the UK was investigated using RFLP, PCR-RFLP and plasmid profile analysis. The results suggest that the four classes are compatible: when two or more different sequences are present in a strain they are usually associated with different plasmids. Furthermore, classical incompatibility studies in which a Tn5-labelled plasmid with a group IV sequence was transferred into field isolates by conjugation demonstrated that group IV sequences are incompatible with each other, but compatible with the other groups. This supports the idea that the different groups represent different incompatibility groups. The same field isolates were also screened for chromosomal (12) and symbiotic gene ( region) variation. Comparison of these and the plasmid data suggest that plasmid transfer does occur within field populations of but that certain plasmid-chromosome combinations are favoured.
运用限制性片段长度多态性分析（RFLP）、聚合酶链反应 - 限制性片段长度多态性分析（PCR - RFLP）以及质粒图谱分析，对法国、德国和英国三个田间群体中的四类相关质粒复制基因（）的分布情况进行了研究。结果表明，这四类是兼容的：当一个菌株中存在两个或更多不同的序列时，它们通常与不同的质粒相关联。此外，经典的不相容性研究中，通过接合作用将带有IV组序列的Tn5标记质粒转移到田间分离株中，结果表明IV组序列彼此不相容，但与其他组兼容。这支持了不同组代表不同不相容组的观点。对相同的田间分离株还进行了染色体（12）和共生基因（区域）变异的筛选。这些数据与质粒数据的比较表明，在的田间群体中确实发生了质粒转移，但某些质粒 - 染色体组合更受青睐。
