Wang Juan, Zhao Hui, Lv Ke, Zhao Wei, Zhang Ning, Yang Fan, Wen Xiang, Jiang Xiaohua, Tian Jingrui, Liu Xinjuan, Ho Chi-Tang, Li Shiming
Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.
Hubei Key Laboratory of EFGIR, Huanggang Normal University, Huanggang, Hubei 438000, China.
J Agric Food Chem. 2021 Apr 7;69(13):3871-3878. doi: 10.1021/acs.jafc.1c00274. Epub 2021 Mar 24.
The integrity of the intestinal barrier is critical for homeostasis. In this study, we investigated the protective effect of pterostilbene (PTE) on the intestinal epithelium barrier. results of transepithelial electrical resistance (TEER) in Caco-2 cells indicated that PTE counteracted tumor necrosis factor α (TNFα)-induced barrier damage. PTE pretreatment markedly ameliorated intestinal barrier dysfunction induced by dextran sulfate sodium (DSS). Notably, intestinal epithelial tight junction (TJ) molecules were restored by PTE in mice exposed to DSS. The mechanism study revealed that PTE prevented myosin light-chain kinase (MLCK) from driving phosphorylation of MLC (p-MLC), which is crucial for maintaining intestinal TJ stability. Furthermore, PTE blunted translocation of NF-κB subunit p65 into the nucleus to downregulate MLCK expression and then to safeguard TJs and barrier integrity. These findings suggest that PTE protected the intestinal epithelial barrier through the NF-κB- MLCK/p-MLC signal pathway.
肠道屏障的完整性对于体内平衡至关重要。在本研究中，我们研究了紫檀芪（PTE）对肠上皮屏障的保护作用。Caco-2细胞的跨上皮电阻（TEER）结果表明，PTE可抵消肿瘤坏死因子α（TNFα）诱导的屏障损伤。PTE预处理显著改善了葡聚糖硫酸钠（DSS）诱导的肠道屏障功能障碍。值得注意的是，在暴露于DSS的小鼠中，PTE可使肠上皮紧密连接（TJ）分子恢复正常。机制研究表明，PTE可阻止肌球蛋白轻链激酶（MLCK）驱动肌球蛋白轻链（p-MLC）磷酸化，而这对于维持肠道TJ稳定性至关重要。此外，PTE可抑制NF-κB亚基p65向细胞核的转位，从而下调MLCK表达，进而保护TJ和屏障完整性。这些发现表明，PTE通过NF-κB-MLCK/p-MLC信号通路保护肠上皮屏障。
