Endocrinology Department, Hebei General Hospital, Shijiazhuang, Hebei 050000, P.R. China.
Mol Med Rep. 2021 May;23(5). doi: 10.3892/mmr.2021.11974. Epub 2021 Mar 24.
Silibinin is a flavonoid that improves fatty liver and insulin resistance. To elucidate the effect of silibinin on lipid deposition and the potential molecular mechanism, the present study conducted and experiments. In the experiments, mice were randomly divided into control, high‑fat and silibinin groups, while HepG2 cells were randomly divided into control, palmitic acid intervention and silibinin intervention groups. The mRNA, protein and miR‑122 expression associated with hepatic lipid metabolism were detected in each group. The results demonstrated that silibinin reduced the triglyceride content, miR‑122 expression and the mRNA and protein expressions of fatty acid synthase (FAS) and acetyl‑CoA carboxylase (ACC). Silibinin increased the mRNA and protein expression of carnitine palmitoyl transferase 1A (CPT1A). In the present study, HepG2 cells cultured with palmitate were treated with silibinin following overexpression of micro RNA (miR) 122. The results demonstrated that the mRNA and protein expression of FAS and ACC was increased, while that of CPT1A was decreased. Therefore, it could be deduced that silibinin improved lipid metabolism by reducing the expression of miR‑122 and inhibiting the expression of miR‑122 may be a new therapeutic target to improve fatty liver disease.
水飞蓟宾是一种改善脂肪肝和胰岛素抵抗的类黄酮。为了阐明水飞蓟宾对脂质沉积的影响及其潜在的分子机制，本研究进行了 和 实验。在 实验中，将小鼠随机分为对照组、高脂组和水飞蓟宾组，而 HepG2 细胞则随机分为对照组、软脂酸干预组和水飞蓟宾干预组。检测各组与肝脂代谢相关的 mRNA、蛋白和 miR-122 表达。结果表明，水飞蓟宾降低了甘油三酯含量、miR-122 表达以及脂肪酸合成酶（FAS）和乙酰辅酶 A 羧化酶（ACC）的 mRNA 和蛋白表达。水飞蓟宾增加了肉毒碱棕榈酰转移酶 1A（CPT1A）的 mRNA 和蛋白表达。在本研究中，用软脂酸培养的 HepG2 细胞在过表达 micro RNA（miR）122 后用水飞蓟宾处理。结果表明，FAS 和 ACC 的 mRNA 和蛋白表达增加，而 CPT1A 的表达减少。因此，可以推断出水飞蓟宾通过降低 miR-122 的表达来改善脂质代谢，抑制 miR-122 的表达可能是改善脂肪肝疾病的新治疗靶点。
