Division of Neurosurgery, University of Saskatchewan, Saskatoon, Canada.
Dept. Pharmacology, University of Saskatchewan, Saskatoon, Canada.
Brain Inj. 2021 May 12;35(6):734-741. doi: 10.1080/02699052.2021.1900602. Epub 2021 Mar 24.
Cellular prion protein (PrPC) is a lipid raft protein abundant within CNS. It is regulated by a disintegrin and metalloproteinase domain containing protein 10 (ADAM10). PrPC has previously been implicated as a biomarker for TBI. ADAM10 has not been investigated as a TBI biomarker.
We evaluated PrPC and ADAM10 as candidate biomarkers for TBI.
We performed ELISA for ADAM10 and PrPC on plasma samples of patients with TBI admitted to Brigham and Women's Hospital. Plasma samples from 20 patients admitted for isolated TBI were acquired from a biobank with clinical information. Control plasma (37 samples) was acquired from a commercial source. GraphPad was used to conduct statistical analysis.
37 controls and 20 TBI samples were collected. Of the patients with TBI, eight were mild, three were moderate, and nine were severe. Both PrPC and ADAM10 were elevated in patients with TBI compared with control ( < .001). ADAM10 exhibited greater expression in patients with worse clinical grade. There was no significant association of either PrPC or ADAM10 with time after injury.
Our results indicate that PrPC and ADAM10 appear to be useful potential tools for screening of TBI. ADAM10 is closely associated with clinical grade.
细胞朊蛋白（PrPC）是中枢神经系统中丰富的脂筏蛋白，受包含金属蛋白酶结构域的去整合素蛋白 10（ADAM10）调节。PrPC 先前被认为是 TBI 的生物标志物。ADAM10 尚未被作为 TBI 生物标志物进行研究。
我们评估 PrPC 和 ADAM10 作为 TBI 的候选生物标志物。
我们在收入布莱根妇女医院的 TBI 患者的血浆样本中进行了 ADAM10 和 PrPC 的 ELISA 检测。从生物银行获得了 20 名因孤立性 TBI 入院的患者的血浆样本，这些样本具有临床信息。从商业来源获得了 37 份对照血浆样本。GraphPad 用于进行统计分析。
收集了 37 名对照者和 20 名 TBI 样本。TBI 患者中，8 例为轻度，3 例为中度，9 例为重度。与对照组相比，TBI 患者的 PrPC 和 ADAM10 均升高（<0.001）。ADAM10 在临床分级较差的患者中表达更高。PrPC 或 ADAM10 与损伤后时间均无显著相关性。
我们的结果表明，PrPC 和 ADAM10 似乎是筛查 TBI 的有用潜在工具。ADAM10 与临床分级密切相关。
