Escuela de Nutrición, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET, Instituto de Investigaciones en Ciencias de la Salud, Córdoba, Argentina.
Facultad de Matemáticas, Astronomía y Física, Universidad Nacional de Córdoba, Córdoba, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET, Instituto de Física "Enrique Gaviola", Córdoba, Argentina.
Biomedica. 2021 Mar 19;41(1):99-110. doi: 10.7705/biomedica.5485.
Introduction: Arsenic is an environmental toxic present worldwide. In men and animals, various organs and tissues are targets of its deleterious effects including those of the immune system. Objective: To determine acute arsenic toxicity in tissues and target cells of Balb/c mice using an in vivo methodology. Materials and methods: We injected Balb/c mice intraperitoneally with 9.5 or 19 mg/kg of sodium arsenite (NaAsO2), or an equivalent volume of physiological solution as a control (with 3 per experimental group). After 30 minutes, the animals were sacrificed to obtain spleen, thymus, liver, kidneys, and blood. We determined arsenic, polyphenols, and iron concentrations in each sample and we evaluated the oxidative markers (peroxides, advanced products of protein oxidation, and free sulfhydryl groups). In splenocytes from the spleen, cell viability and mitochondrial potential were also determined. Results: The exposure to an acute dose of NaAsO2 reduced the mitochondrial function of splenocytes, which resulted in cell death. Simultaneously, the confirmed presence of arsenic in spleen samples and the resulting cytotoxicity occurred with a decrease in polyphenols, free sulfhydryl groups, and an alteration in the content and distribution of iron, but did not increase the production of peroxides. Conclusion: These findings provide scientific evidence about changes occurring in biomarkers involved in the immunotoxicity of arsenic and offer a methodology for testing possible treatments against the deleterious action of this compound on the immune system.
砷是一种在全球范围内存在的环境毒物。在人和动物中，其有害影响的靶器官和组织包括免疫系统。目的：采用体内方法确定亚砷酸钠（NaAsO2）对 Balb/c 小鼠组织和靶细胞的急性毒性。材料和方法：我们通过腹腔注射向 Balb/c 小鼠注射 9.5 或 19mg/kg 的亚砷酸钠（NaAsO2），或作为对照注射等量的生理盐水（每组实验动物 3 只）。30 分钟后，处死动物以获得脾脏、胸腺、肝脏、肾脏和血液。我们测定了每个样本中的砷、多酚和铁浓度，并评估了氧化标记物（过氧化物、蛋白质氧化的高级产物和游离巯基）。还测定了来自脾脏的脾细胞中的细胞活力和线粒体电势。结果：暴露于急性剂量的 NaAsO2 降低了脾细胞的线粒体功能，导致细胞死亡。同时，在脾样本中确认存在砷以及由此产生的细胞毒性与多酚、游离巯基的减少以及铁的含量和分布改变有关，但没有增加过氧化物的产生。结论：这些发现为砷的免疫毒性相关生物标志物变化提供了科学依据，并提供了一种方法来测试针对这种化合物对免疫系统的有害作用的可能治疗方法。
