对硫磷中毒后的脑乙酰胆碱酯酶：死后比较定量组织化学分析

Brain acetylcholinesterase after parathion poisoning: a comparative quantitative histochemical analysis post-mortem.

作者信息

Finkelstein Y, Wolff M, Biegon A

机构信息

Israeli Poison Control Center, Rambam Medical Center, Haifa, Israel.

出版信息

Toxicology. 1988 Apr;49(1):165-9. doi: 10.1016/0300-483x(88)90189-8.

DOI:10.1016/0300-483x(88)90189-8

PMID:3376122

Abstract

The regional distribution of AChE inhibition by parathion in the human brain was examined in a comparative study of the brains of 2 victims of lethal parathion intoxication and 2 control brains matched for age and sex. AChE activity in discrete brain regions was studied by quantitative histochemistry of 40 microns-thick sagittal or coronal cryostat sections from the 4 brains. The inhibition of human brain AChE by parathion is regionally selective. The biggest decreases were observed in the cerebellum, some thalamic nuclei and cortex. Only a moderate decrease (10-30%) was observed in the substantia nigra and basal ganglia, while no effect at all was seen in white matter regions. Detailed knowledge of the brain regions affected by parathion poisoning may explain some of the clinical manifestations of organophosphate poisoning.

摘要

在一项对2名致死性对硫磷中毒受害者的大脑与2名年龄和性别相匹配的对照大脑进行的比较研究中，检测了对硫磷对人脑中乙酰胆碱酯酶（AChE）抑制作用的区域分布。通过对来自这4个大脑的40微米厚矢状或冠状低温恒温器切片进行定量组织化学，研究了离散脑区中的AChE活性。对硫磷对人脑中AChE的抑制具有区域选择性。在小脑、一些丘脑核和皮质中观察到最大程度的降低。在黑质和基底神经节中仅观察到中度降低（10%-30%），而在白质区域未观察到任何影响。对受对硫磷中毒影响的脑区的详细了解可能有助于解释有机磷中毒的一些临床表现。