Karsauliya Kajal, Bhateria Manisha, Sonker Ashish, Singh Sheelendra Pratap
Pesticide Toxicology Laboratory/Analytical Chemistry Laboratory, Regulatory Toxicology, and Food, Drug & Chemical Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Vishvigyan Bhawan, 31 Mahatma Gandhi Marg, Lucknow 226001, Uttar Pradesh, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
J Agric Food Chem. 2021 Apr 7;69(13):3932-3941. doi: 10.1021/acs.jafc.1c00129. Epub 2021 Mar 24.
Bisphenol A (BPA) is a well-recognized endocrine disruptor, and considering its adverse effects its use in infant bottles has been banned in many countries. Growing concern on the use of BPA has led to its replacement with its analogues in numerous applications. Present is the first report determining the occurrence of seven bisphenols (BPs: BPA, BPAF, BPC, BPE, BPFL, BPS, and BPZ) in Indian infant formula. A reliable and efficient UPLC-MS/MS method for their simultaneous determination was developed and validated in powdered infant formula ( = 68). The limit of quantification of the method was 0.19 ng/g for BPA, BPAF, BPE, BPS and BPZ and 0.78 ng/g for BPC and BPFL. The highest concentration was detected for BPA (mean = 5.46 ng/g) followed by BPZ and BPS. BPAF, BPFL, BPC and BPE were detected in none of the samples. The estimated daily intake (EDI) of total BPs in infants (0-12 months old infants) was determined to be 54.33-213.36 ng/kg b.w./day. BPA mainly contributed to the total intake (EDI = 92.76 ng/kg b.w./day). The dietary exposure to total BPs evaluated in the present study was approximately 1 order of magnitude lower than the reference value of BPA set by EFSA (4 μg/kg b.w./day) and, thus, may not pose considerable risks to infants.
双酚A（BPA）是一种广为人知的内分泌干扰物，鉴于其不良影响，许多国家已禁止在婴儿奶瓶中使用。人们对双酚A使用的日益关注导致其在众多应用中被其类似物所取代。本文首次报道了在印度婴儿配方奶粉中检测到七种双酚（BPs：双酚A、双酚AF、双酚C、双酚E、双酚FL、双酚S和双酚Z）。开发了一种可靠且高效的超高效液相色谱-串联质谱法（UPLC-MS/MS）用于同时测定粉状婴儿配方奶粉（n = 68）中的这些双酚，并进行了方法验证。该方法对双酚A、双酚AF、双酚E、双酚S和双酚Z的定量限为0.19 ng/g，对双酚C和双酚FL的定量限为0.78 ng/g。检测到双酚A的浓度最高（平均值 = 5.46 ng/g），其次是双酚Z和双酚S。在所有样品中均未检测到双酚AF、双酚FL、双酚C和双酚E。测定了婴儿（0至12个月大的婴儿）中总双酚的估计每日摄入量（EDI）为54.33 - 213.36 ng/kg体重/天。双酚A是总摄入量的主要贡献者（EDI = 92.76 ng/kg体重/天）。本研究评估的婴儿通过饮食摄入的总双酚比欧洲食品安全局（EFSA）设定的双酚A参考值（4 μg/kg体重/天）低约1个数量级，因此可能不会对婴儿构成重大风险。
