NanoTechnas - Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko str. 24, 03225 Vilnius, Lithuania; State Research Institute Center for Physical and Technological Sciences, Sauletekio ave. 3, Vilnius, Lithuania.
NanoTechnas - Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko str. 24, 03225 Vilnius, Lithuania.
J Colloid Interface Sci. 2021 Jul 15;594:195-203. doi: 10.1016/j.jcis.2021.02.100. Epub 2021 Mar 10.
During the pandemic, different methods for SARS-CoV-2 detection and COVID-19 diagnostics were developed, including antibody and antigen tests. For a better understanding of the interaction mechanism between SARS-CoV-2 virus proteins and specific antibodies, total internal reflection ellipsometry based evaluation of the interaction between SARS-CoV-2 nucleoprotein (SCoV2-rN) and anti-SCoV2-rN antibodies was performed. Results show that the appropriate mathematical model, which takes into account the formation of an intermediate complex, can be applied for the evaluation of SCoV2-rN/anti-SCoV2-rN complex formation kinetics. The calculated steric factor indicated that SCoV2-rN/anti-SCoV2-rN complex formation has very strict steric requirements. Estimated Gibbs free energy (ΔG) for SCoV-rN and anti-SCoV-rN binding was determined as -34 kJ/mol. The reported findings are useful for the design of new analytical systems for the determination of anti-SCoV2-rN antibodies and for the development of new anti-SARS-CoV-2 medications.
在大流行期间，开发了用于 SARS-CoV-2 检测和 COVID-19 诊断的不同方法，包括抗体和抗原检测。为了更好地了解 SARS-CoV-2 病毒蛋白与特定抗体之间的相互作用机制，利用全内反射椭圆术评估了 SARS-CoV-2 核蛋白（SCoV2-rN）与抗 SCoV2-rN 抗体之间的相互作用。结果表明，可以应用适当的数学模型（考虑到中间复合物的形成）来评估 SCoV2-rN/抗 SCoV2-rN 复合物形成的动力学。计算得到的空间位阻因子表明，SCoV2-rN/抗 SCoV2-rN 复合物的形成具有非常严格的空间位阻要求。确定 SCoV-rN 和抗 SCoV-rN 结合的吉布斯自由能（ΔG）为-34 kJ/mol。报道的研究结果对于设计用于测定抗 SCoV2-rN 抗体的新分析系统以及开发新的抗 SARS-CoV-2 药物非常有用。
