Shulan (Hangzhou) Hospital Affiliated to Zhejiang Shuren University, Shulan International Medical College.
Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, School of Medicine.
Medicine (Baltimore). 2021 Mar 26;100(12):e25320. doi: 10.1097/MD.0000000000025320.
To investigate the expression pattern and diagnostic performance of matrix metalloproteinase 28 (MMP28) in pancreatic cancer (PC).The RNA-seq data of PC and normal pancreas tissue were acquired from The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression. Clinical information of PC that included prognostic data was obtained from TCGA. Later, Fisher exact test was applied for comparison of different clinicopathological features between high and low expression of MMP28 in PC. Afterwards, Kaplan-Meier survival analysis and Cox analysis (univariate and multivariate analysis) were used to explore the prognostic performance of MMP28 in PC cohort. Finally, gene set enrichment analysis (GSEA) revealed the potential signaling pathways related to high expression of MMP28 in PC.Upregulation of MMP28 was identified in PC tissue compared to normal pancreas tissue (P < .001). Overexpression of MMP28 was related to histological grade (P < .001), M classification (P = .014), and survival status (P = .028). Kaplan-Meier survival analysis revealed that high level of MMP28 implied unfavorable prognosis in PC (P = .002). Multivariate analysis confirmed that MMP28 was an independent risk factor in PC (hazard rate = 1.308, P = .018). Our GSEA analysis found that signaling pathways including glycolysis, p53 pathway, notch signaling, estrogen response late, cholesterol homeostasis, estrogen response early, mitotic spindle, and transforming growth factor beta signaling were enriched in the group with higher MMP28 expression.High expression of MMP28 could be identified in PC, which also served as an independent risk element for PC.
为了研究基质金属蛋白酶 28（MMP28）在胰腺癌（PC）中的表达模式和诊断性能。我们从癌症基因组图谱（TCGA）和基因型组织表达中获取了 PC 和正常胰腺组织的 RNA-seq 数据。从 TCGA 获得了包含预后数据的 PC 临床信息。之后，应用 Fisher 确切检验比较 PC 中 MMP28 高表达和低表达的不同临床病理特征。随后，进行 Kaplan-Meier 生存分析和 Cox 分析（单因素和多因素分析）以探讨 MMP28 在 PC 队列中的预后性能。最后，基因集富集分析（GSEA）揭示了与 PC 中 MMP28 高表达相关的潜在信号通路。与正常胰腺组织相比，PC 组织中 MMP28 的表达上调（P<0.001）。MMP28 的过表达与组织学分级（P<0.001）、M 分类（P=0.014）和生存状态（P=0.028）有关。Kaplan-Meier 生存分析显示，MMP28 高水平提示 PC 预后不良（P=0.002）。多因素分析证实 MMP28 是 PC 的独立危险因素（危险比=1.308，P=0.018）。我们的 GSEA 分析发现，在 MMP28 高表达组中，包括糖酵解、p53 途径、Notch 信号、雌激素反应晚期、胆固醇稳态、雌激素反应早期、有丝分裂纺锤体和转化生长因子β信号在内的信号通路富集。MMP28 在 PC 中表达升高，可作为 PC 的独立危险因素。
