Nash Family Department of Neuroscience and Friedman Brain Institute, New York, USA.
Department of Genetics and Genomic Sciences and Icahn Institute for Data Science and Genomics Technology, Icahn School of Medicine At Mount Sinai, New York, NY, 10029, USA.
Sci Rep. 2021 Mar 24;11(1):6729. doi: 10.1038/s41598-021-86068-7.
To better understand the full-length transcriptome of the nucleus accumbens (NAc)-a key brain reward region-in chronic cocaine treatment, we perform the first single molecule, long-read sequencing analysis using the Iso-seq method to detect 42,114 unique transcripts from mouse NAc polyadenylated RNA. Using GENCODE annotation as a reference, we find that over half of the Iso-seq derived transcripts are annotated, while 46% of them harbor novel splicing events in known genes; around 1% of them correspond to other types of novel transcripts, such as fusion, antisense and intergenic. Approximately 34% of the novel transcripts are matched with a compiled transcriptome assembled from published short-read data from various tissues, with the remaining 69% being unique to NAc. These data provide a more complete picture of the NAc transcriptome than existing annotations and can serve as a comprehensive reference for future transcriptomic analyses of this important brain reward region.
为了更全面地了解伏隔核（NAc）的全长转录组，伏隔核是大脑奖赏区域的关键部位，我们首次采用 Iso-seq 方法对其进行单分子、长读长测序分析，检测到来自小鼠 NAc 多聚腺苷酸化 RNA 的 42114 个独特转录本。利用 GENCODE 注释作为参考，我们发现 Iso-seq 衍生的转录本中有一半以上被注释，而其中 46%的转录本在已知基因中存在新的剪接事件；约 1%的转录本对应其他类型的新型转录本，如融合、反义及基因间转录本。大约 34%的新型转录本与来自不同组织的已发表短读数据组装的编译转录组匹配，而其余 69%的转录本是 NAc 所特有的。这些数据比现有注释提供了更全面的 NAc 转录组图谱，可作为该重要脑奖赏区域未来转录组分析的综合参考。
