Sun Jialing, Chen Weicong, Wen Bin, Zhang Mingjia, Sun Haitao, Yang Xuemei, Zhao Wenting, La Lei, An Haiyan, Pang Jie, Gao Lei, He Songqi
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.
Department of Traditional Chinese Medicine, The Air Force Hospital of Southern Theater Command, Guangzhou, China.
Front Pharmacol. 2021 Mar 8;12:610158. doi: 10.3389/fphar.2021.610158. eCollection 2021.
Hepatocellular carcinoma (HCC) is among the most usual cancers globally. In China, Biejiajian pill (BJJP), Traditional Chinese Medicine clinical prescription, is broadly utilized for the prevention and therapy of HCC. However, the mechanisms by which BJJP exerts its effects on the prevention of tumor invasion and metastasis are still largely unknown. In this study, multiple hepatic cancer cell lines and an xenograft mice model were used to validate the preventive effects and molecular mechanisms of BJJP in HCC. We established that BJJP significantly repressed the proliferation, metastasis and infiltration of HCC cells. Furthermore, BJJP remarkably suppressed HCC cell migration, as well as invasion via epithelial-mesenchymal transition (EMT) by modulating Snail expression, which was associated with the repression of Akt/GSK-3β/Snail signaling axis activation. HCC xenograft results indicated that BJJP delayed HCC development and efficiently inhibited lung metastasis. Taken together, BJJP was shown to be an effective therapeutic agent against HCC through repression of the Akt/GSK-3β/Snail signaling cascade and EMT.
肝细胞癌（HCC）是全球最常见的癌症之一。在中国，中药临床方剂鳖甲煎丸（BJJP）被广泛用于HCC的预防和治疗。然而，BJJP发挥其预防肿瘤侵袭和转移作用的机制仍 largely未知。在本研究中，使用多种肝癌细胞系和异种移植小鼠模型来验证BJJP在HCC中的预防作用和分子机制。我们发现BJJP显著抑制HCC细胞的增殖、转移和浸润。此外，BJJP通过调节Snail表达显著抑制HCC细胞迁移以及上皮-间质转化（EMT）介导的侵袭，这与抑制Akt/GSK-3β/Snail信号轴激活有关。HCC异种移植结果表明，BJJP延缓了HCC的发展并有效抑制了肺转移。综上所述，BJJP通过抑制Akt/GSK-3β/Snail信号级联反应和EMT被证明是一种有效的抗HCC治疗药物。
