Institute for Research in Biomedicine - IRB Barcelona, the Barcelona Institute for Science and Technology - BIST, Barcelona, Spain.
Department of Developmental Biology, Instituto de Biología Molecular de Barcelona, CSIC, Barcelona, Spain.
F1000Res. 2020 Nov 10;9:1308. doi: 10.12688/f1000research.27112.2. eCollection 2020.
We introduce MosaicExplorerJ, an ImageJ macro to stitch 3D tiles from terabyte-size microscopy datasets organized on a regular 2D grid. As opposed to existing software, stitching does not require any prior information on the actual positions of the tiles, or conversion of raw TIFF images to a multi-resolution format for interactive exploration and fast processing. MosaicExplorerJ was specifically designed to process lightsheet microscopy datasets from optically cleared samples. It can handle multiple fluorescence channels, dual-sided lightsheet illumination and dual-sided camera detection.
我们介绍了 MosaicExplorerJ，这是一个 ImageJ 宏，用于拼接在规则 2D 网格上组织的 TB 级显微镜数据集的 3D 瓦片。与现有软件不同，拼接不需要任何关于瓦片实际位置的先验信息，也不需要将原始 TIFF 图像转换为用于交互式探索和快速处理的多分辨率格式。MosaicExplorerJ 是专门为处理经过光透明化处理的样本的光片显微镜数据集而设计的。它可以处理多个荧光通道、双面光片照明和双面相机检测。
