Chroumpi Tania, Peng Mao, Markillie Lye Meng, Mitchell Hugh D, Nicora Carrie D, Hutchinson Chelsea M, Paurus Vanessa, Tolic Nikola, Clendinen Chaevien S, Orr Galya, Baker Scott E, Mäkelä Miia R, de Vries Ronald P
Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Utrecht, Netherlands.
Environmental Molecular Science Laboratory, Pacific Northwest National Laboratory, Richland, WA, United States.
Front Bioeng Biotechnol. 2021 Mar 8;9:644216. doi: 10.3389/fbioe.2021.644216. eCollection 2021.
The filamentous ascomycete has received increasing interest as a cell factory, being able to efficiently degrade plant cell wall polysaccharides as well as having an extensive metabolism to convert the released monosaccharides into value added compounds. The pentoses D-xylose and L-arabinose are the most abundant monosaccharides in plant biomass after the hexose D-glucose, being major constituents of xylan, pectin and xyloglucan. In this study, the influence of selected pentose catabolic pathway (PCP) deletion strains on growth on plant biomass and re-routing of sugar catabolism was addressed to gain a better understanding of the flexibility of this fungus in using plant biomass-derived monomers. The transcriptome, metabolome and proteome response of three PCP mutant strains, ΔΔΔ, ΔΔΔ and Δ, grown on wheat bran (WB) and sugar beet pulp (SBP), was evaluated. Our results showed that despite the absolute impact of these PCP mutations on pure pentose sugars, they are not as critical for growth of on more complex biomass substrates, such as WB and SBP. However, significant phenotypic variation was observed between the two biomass substrates, but also between the different PCP mutants. This shows that the high sugar heterogeneity of these substrates in combination with the high complexity and adaptability of the fungal sugar metabolism allow for activation of alternative strategies to support growth.
丝状子囊菌作为一种细胞工厂受到了越来越多的关注，它能够有效降解植物细胞壁多糖，并且具有广泛的代谢能力，可将释放出的单糖转化为高附加值化合物。戊糖D-木糖和L-阿拉伯糖是继己糖D-葡萄糖之后植物生物质中含量最丰富的单糖，是木聚糖、果胶和木葡聚糖的主要成分。在本研究中，研究了选定的戊糖分解代谢途径（PCP）缺失菌株对植物生物质生长的影响以及糖分解代谢的重新定向，以更好地了解这种真菌在利用植物生物质衍生单体方面的灵活性。评估了在麦麸（WB）和甜菜粕（SBP）上生长的三种PCP突变菌株ΔΔΔ、ΔΔΔ和Δ的转录组、代谢组和蛋白质组反应。我们的结果表明，尽管这些PCP突变对纯戊糖有绝对影响，但它们对在更复杂的生物质底物（如WB和SBP）上的生长并非至关重要。然而，在两种生物质底物之间以及不同的PCP突变体之间都观察到了显著的表型差异。这表明这些底物的高糖异质性与真菌糖代谢的高复杂性和适应性相结合，使得能够激活替代策略来支持生长。
