Chroumpi Tania, Peng Mao, Aguilar-Pontes Maria Victoria, Müller Astrid, Wang Mei, Yan Juying, Lipzen Anna, Ng Vivian, Grigoriev Igor V, Mäkelä Miia R, de Vries Ronald P
Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, Utrecht, 3584 CT, The Netherlands.
US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA, 94720, USA.
Microb Biotechnol. 2021 Nov;14(6):2525-2537. doi: 10.1111/1751-7915.13790. Epub 2021 Mar 5.
Next to d-glucose, the pentoses l-arabinose and d-xylose are the main monosaccharide components of plant cell wall polysaccharides and are therefore of major importance in biotechnological applications that use plant biomass as a substrate. Pentose catabolism is one of the best-studied pathways of primary metabolism of Aspergillus niger, and an initial outline of this pathway with individual enzymes covering each step of the pathway has been previously established. However, although growth on l-arabinose and/or d-xylose of most pentose catabolic pathway (PCP) single deletion mutants of A. niger has been shown to be negatively affected, it was not abolished, suggesting the involvement of additional enzymes. Detailed analysis of the single deletion mutants of the known A. niger PCP genes led to the identification of additional genes involved in the pathway. These results reveal a high level of complexity and redundancy in this pathway, emphasizing the need for a comprehensive understanding of metabolic pathways before entering metabolic engineering of such pathways for the generation of more efficient fungal cell factories.
除了d-葡萄糖外，戊糖l-阿拉伯糖和d-木糖是植物细胞壁多糖的主要单糖成分，因此在以植物生物质为底物的生物技术应用中具有重要意义。戊糖分解代谢是黑曲霉初级代谢中研究最深入的途径之一，此前已经建立了该途径的初步轮廓，其中涵盖了该途径每个步骤的各个酶。然而，尽管已表明黑曲霉大多数戊糖分解代谢途径（PCP）单缺失突变体在l-阿拉伯糖和/或d-木糖上的生长受到负面影响，但并未完全消除，这表明还有其他酶参与其中。对已知黑曲霉PCP基因的单缺失突变体进行详细分析，从而鉴定出该途径中涉及的其他基因。这些结果揭示了该途径的高度复杂性和冗余性，强调了在对这些途径进行代谢工程以生成更高效的真菌细胞工厂之前，全面了解代谢途径的必要性。
