大鼠实验性草酸中毒：一项电子显微镜研究。

Experimental oxalosis in rats: an electron microscopic study.

作者信息

Mijan J L, Aneiros J, O'Valle F, Zuloaga A, Martinez J L, Camara M

机构信息

Department of Urology, University of Granada, Spain.

出版信息

Urol Int. 1988;43(1):19-23. doi: 10.1159/000281296.

DOI:10.1159/000281296

PMID:3376358

Abstract

The ultrastructural change in rat kidneys was analyzed after intraperitoneal injection of oxalic acid. This study demonstrates fundamental alterations in the epithelial cells of the proximal convoluted tubules (PCT). At 8 h (group IV), cytoplasmic prolongations (CP) begin to be observed, which fill the lumens of PCT at 20 h (group VII) and 24 h (group VIII). Unusual mechanism of cytoplasmic secretion is suggested to this fact. Calcium salts were found on CP and in group VIII. At 16 h (group IV), intracitoplasmic membranous structures (MS) appear, which can emigrate across the microvilli into the lumens of PCT. At 24 h (group VIII), MS are also shown in basal zone and in basement membrane. We considered that CP, which can fragment, and MS are the ultrastructural substratum of the matrix of the calculis, corresponding probably to the substance A.

摘要

腹腔注射草酸后分析大鼠肾脏的超微结构变化。本研究表明近端曲管（PCT）上皮细胞发生了根本性改变。在8小时（IV组）时，开始观察到细胞质延长物（CP），其在20小时（VII组）和24小时（VIII组）时充满PCT管腔。针对这一事实提出了细胞质分泌的异常机制。在CP上以及VIII组中发现了钙盐。在16小时（IV组）时，出现了胞质内膜性结构（MS），其可穿过微绒毛迁移到PCT管腔中。在24小时（VIII组）时，MS也出现在基底区和基底膜中。我们认为，可断裂的CP和MS是结石基质的超微结构基础，可能对应于物质A。