Hallén-Sandgren C, Björk I
Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences, Uppsala Biomedical Center.
Vet Immunol Immunopathol. 1988 Feb;18(1):81-94. doi: 10.1016/0165-2427(88)90038-4.
A new method for the isolation of bovine neutrophilic granulocytes from peripheral blood based on centrifugation in a discontinuous metrizamide gradient has been developed. The procedure is rapid, taking only about 2 h, and gives highly purified (greater than 90%) neutrophils in a high yield (approximately 85%). The function of the cells, as measured by chemiluminescence and migration assays, is not significantly influenced by the isolation procedure. Eosinophils can also be isolated by a slight variation of the method. Initial applications of the separation procedure indicate its usefulness in clinical studies of bovine neutrophil function. A variation between individuals in the function of the cells was thus demonstrated. Moreover, the chemiluminescence of neutrophils from infected animals was found to be greatly increased, and neutrophil migration was shown to be stimulated by in vivo ACTH treatment.
已开发出一种基于在不连续的甲泛影酰胺梯度中离心从外周血中分离牛嗜中性粒细胞的新方法。该过程快速，仅需约2小时，并且能以高产量（约85%）获得高度纯化（大于90%）的嗜中性粒细胞。通过化学发光和迁移试验测定，细胞功能不受分离过程的显著影响。嗜酸性粒细胞也可通过该方法的轻微改动进行分离。该分离程序的初步应用表明其在牛嗜中性粒细胞功能临床研究中的有用性。由此证明了细胞功能在个体之间存在差异。此外，发现感染动物的嗜中性粒细胞化学发光大大增加，并且体内促肾上腺皮质激素治疗可刺激嗜中性粒细胞迁移。
