Chambers W H, Washburn S M, Campos M, Klesius P H
Am J Vet Res. 1985 Jan;46(1):154-6.
A method for the isolation of an enriched population (greater than 60%) of bovine eosinophils was developed, using density gradient centrifugation. Eosinophils were isolated from peripheral blood by centrifugation on a density gradient medium of sp gr 1.092. Viability was greater than or equal to 95%, and the isolated eosinophils were found to be functional in chemokinetic and antibody-dependent cellular-cytotoxicity assays. This method represents a simple, rapid method for obtaining bovine eosinophils that can be used in a variety of assays of eosinophil function.
采用密度梯度离心法，开发了一种分离富含牛嗜酸性粒细胞群体（大于60%）的方法。通过在比重为1.092的密度梯度介质上离心，从外周血中分离嗜酸性粒细胞。存活率大于或等于95%，并且发现分离出的嗜酸性粒细胞在化学动力学和抗体依赖性细胞毒性试验中具有功能。该方法是一种简单、快速获得牛嗜酸性粒细胞的方法，可用于多种嗜酸性粒细胞功能检测。
